Electro-optical device and methods for hybridization and detection
AssigneeAgilent Technologies, Inc.
InventorsGanapati R. Mauze, Dan-Hui Yang
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Abstract
The invention provides an apparatus and method for detection of a target molecule. The apparatus includes a probe labeled with a transition metal-ligand complex that hybridizes with the target to form an initial complex, a metal ion for doping the initial complex and forming a final complex, and a potential means for providing a potential to the final complex to produce a detectable signal indicating the presence of the target after redox reaction. The method of the invention teaches the steps of hybridizing a probe with an attached label to the target to produce an initial complex, adding a metal ion to the initial complex to form a final complex and applying a potential to the final complex to produce a measurable signal.
Claims21
An apparatus for detecting the presence of a target, comprising:
- (a) a probe labeled with a transition metal-ligand complex, said probe for hybridizing with said target to form an initial complex;
- (b) a metal ion for doping the initial complex and forming a final complex;
- and
- (c) potential means for providing a potential to said final complex to produce a detectable signal indicating the presence of said target.
A method for detecting the presence of a target, comprising:
- (a) hybridizing a probe with an attached label to said target to produce an initial complex;
- (b) adding a metal ion to the initial complex to produce a final complex;
- and
- (c) applying a potential to the final complex to produce a measurable signal.
A method for detecting the presence of a target, comprising adding together a probe having an attached label, a target capable of hybridizing to the probe, and metal ions.
A method for detecting the presence of a target, comprising:
- (a) hybridizing a probe having an attached label with said target to produce an initial complex, wherein the label produces a signal in response to application of a potential;
- (b) adding a metal ion to the initial complex to produce a final electrically conductive complex;
- and
- (c) applying the potential through the final complex to the label, to cause the label to produce the signal.
A kit for detecting the presence of a target, comprising:
- (a) a probe having an attached label that hybridizes to said target to produce an initial complex, wherein the label produces a signal in response to application of a potential;
- (b) a metal ion for adding to the initial complex to produce a final electrically conductive complex;
- and
- (c) potential means for applying a potential through said final complex to said label, to cause the label to produce a signal.
Dependencies are inferred from the claim text.