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US 2002/0164587 A1Published application
Methods for detecting interaction among several proteins
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Centre National De La Recherche Scientifique, Bristol-Myers Squibb Company
InventorsJACQUES CAMONIS, FRANCISCO ROMERO-PORTILLO, RICARDO ATTAR, JEAN-MARC EGLY, FRANCK TIRODE
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Abstract
The aim of the invention is to use at least one conditional promoter to implement a method for detecting one or more proteins interacting directly or indirectly with at least two other proteins during the formation of a protein complex, or during the inhibition of the formation of a protein complex.
Claims9
Method for detecting a protein interacting directly or indirectly with at least two given proteins, characterized in that it comprises:
- a step of transforming host cells whose genome contains one or more reporter genes with one or more vectors such that the genome of these host cells contains:
- on the one hand, the DNA sequence encoding the said test protein capable of interacting with at least two given proteins, the transcription of this DNA sequence being placed under the control of a conditional promoter, and
- on the other hand, the DNA sequences encoding the said given proteins,
- a comparison of the effects of repression of the conditional promoter with the effects of activation of this conditional promoter on the possible transcription of the abovementioned reporter gene(s), it being possible to correlate the results of this comparison with the detection of an interaction or, on the contrary, the absence of an interaction between the said test protein and the said given proteins.
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Dependencies are inferred from the claim text.