Ebola virion proteins expressed from venezuelan equine encephalitis (vee) virus replicons
OwnerMary K. Hart, Julie A. Wilson +3inventors
InventorsMARY K. HART, JULIE A. WILSON, PETER PUSHKO, JONATHAN F. SMITH, ALAN L. SCHMALJOHN
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Abstract
Using the Ebola GP, NP, VP24, VP30, VP35 and VP40 virion proteins, a method and composition for use in inducing an immune response which is protective against infection with Ebola virus is described.
Claims60
A DNA fragment which encodes a GP Ebola protein, said DNA fragment comprising the sequence specified in SEQ ID NO:1, or a polynucleotide fragment comprising at least 15 nucleotides.
A DNA fragment which encodes a NP Ebola protein, said DNA fragment comprising the sequence specified in SEQ ID NO:2, or a polynucleotide fragment comprising at least 15 nucleotides.
A DNA fragment which encodes a VP24 Ebola protein, said DNA fragment comprising the sequence specified in SEQ ID NO:3, or a polynucleotide fragment comprising at least 15 nucleotides.
A DNA fragment which encodes a VP30 Ebola protein, said DNA fragment comprising the sequence specified in any of SEQ ID NO:4 and SEQ ID NO:7, or a polynucleotide fragment comprising at least 15 nucleotides.
A DNA fragment which encodes a VP35 Ebola protein, said DNA fragment comprising the sequence specified in SEQ ID NO:5, or a polynucleotide fragment comprising at least 15 nucleotides.
A DNA fragment which encodes a VP40 Ebola protein, said DNA fragment comprising the sequence specified in SEQ ID NO:6, or a polynucleotide fragment comprising at least 15 nucleotides.
A DNA fragment which encodes a GP Ebola protein said DNA fragment comprising a DNA sequence encoding at least 5 amino acids specified in SEQ ID NO:17 or a conservative substitution thereof.
A DNA fragment which encodes a NP Ebola protein said DNA fragment comprising a DNA sequence encoding at least 5 amino acids specified in SEQ ID NO:18 or a conservative substitution thereof.
A DNA fragment which encodes a VP24 Ebola protein said DNA fragment comprising a DNA sequence encoding at least 5 amino acids specified in SEQ ID NO:19 or a conservative substitution thereof.
A DNA fragment which encodes a VP30 Ebola protein said DNA fragment comprising a DNA sequence encoding at least 5 amino acids specified in any of SEQ ID NO:20 and SEQ ID NO:23 or a conservative substitution thereof.
A DNA fragment which encodes a VP35 Ebola protein said DNA fragment comprising a DNA sequence encoding at least 5 amino acids specified in SEQ ID NO:21 or a conservative substitution thereof.
A DNA fragment which encodes a VP40 Ebola protein said DNA fragment comprising a DNA sequence encoding at least 5 amino acids specified in SEQ ID NO:22 or a conservative substitution thereof.
A recombinant DNA construct comprising:
- (i) a vector, and
- (ii) at least one of the Ebola virus DNA fragments chosen from the group consisting of SEQ ID NO:1, 2, 3, 4, 5, 6 and 7 or a fragment thereof comprising at least 15 nucleotides.
A recombinant DNA construct comprising:
- (i) a vector, and
- (ii) at least one of the Ebola virus DNA fragments chosen from the group consisting of SEQ ID NO:
- 17, 18, 19, 20, 21, 22, 23, 24 and 25 or a conservative substitution thereof.
Self replicating RNA produced from a construct chosen from the group consisting of EboVP24ReP, EboVP30ReP, EboVP35ReP, EboVP40ReP, EboVPNPReP, EboVPGPReP, and EboVP30ReP(#2).
An isolated and purified Ebola GP protein specified in SEQ ID NO:17 and conservative substitutions thereof, or an immunologically identifiable portion thereof.
An isolated and purified Ebola NP protein specified in SEQ ID NO:18 and conservative substitutions thereof or an immunologically identifiable portion thereof.
An isolated and purified Ebola VP24 protein specified in SEQ ID NO:19 and conservative substitutions thereof or an immunologically identifiable portion thereof.
An isolated and purified Ebola VP30 protein specified in any of SEQ ID NO:20 and SEQ ID NO:23 and conservative substitutions thereof or an immunologically identifiable portion thereof.
An isolated and purified Ebola VP35 protein specified in SEQ ID NO:21 and conservative substitutions thereof or an immunologically identifiable portion thereof.
An isolated and purified Ebola VP40 protein specified in SEQ ID NO:22 and conservative substitutions thereof or an immunologically identifiable portion thereof.
An antibody to a peptide encoded by the sequence specified in SEQ ID NO:17, 18, 19, 20, 21, 22, 23, 24, and 25.
A method for detecting the presence or absence of Ebola virus GP RNA in a sample using the polymerase chain reaction using primers for Ebola GP nucleic acid sequence specified in SEQ ID NO:1 for GP.
An Ebola infection diagnostic kit comprising at least 12 consecutive nucleotides of SEQ ID NO:1 specific for the amplification of DNA or RNA of Ebola virus in a sample using the polymerase chain reaction and ancillary reagents suitable for use in such a reaction for detecting the presence or absence of Ebola virus DNA or RNA in a sample.
A pharmaceutical composition comprising one or more recombinant DNA constructs chosen from the group consisting of VRepEboVP24, VRepEboVP30, VRepEboVP35, VRepEboVP40, VRepEboNP, VRepEboGP, and VRepEboVP30(#2), in a pharmaceutically acceptable amount, in a pharmaceutically accpetable carrier and/or adjuvant.
A pharmaceutical composition comprising comprising a peptide encoded by any of SEQ ID NO:24 and SEQ ID NO:25, in a pharmaceutically acceptable amount, in a pharmaceutically acceptable carrier and/or adjuvant.
Dependencies are inferred from the claim text.