Methods for identifying compounds that do not attenuate the protective effects of estrogen
OwnerChawnshang Changinventor
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Abstract
Disclosed are compositions and methods for determining whether a compound attenuates the protection an estrogen confers on a population of cells.
Claims29
A method for determining whether a compound modulates the protection an estrogen confers on a population of cells, the method comprising
- a) exposing the cells to the compound
- b) exposing the cells to the estrogen,
- c) exposing the cells with an agent that damages the cells, and
- d) evaluating the effect of estrogen on the cells in the presence of the compound, wherein a change in the effect of estrogen on the cells, relative to that within a population of cells that has not been exposed to the compound, indicates that the compound modulates the protection an estrogen confers on a population of cells.
A method for determining whether a compound attenuates the protection an estrogen confers on a population of cells, the method comprising
- a) exposing the cells to the compound and the estrogen,
- b) treating the cells with an agent that generates a reactive oxygen species in the cells, and
- c) evaluating survival of the cells, wherein a decrease in the number of surviving cells, relative to that within a population of cells that has not been exposed to the compound, indicates that the compound attenuates the protection an estrogen confers on a population of cells.
A method for determining whether a compound attenuates the protection an estrogen confers on a population of cells, the method comprising
- a) exposing the cells to the compound and the estrogen,
- b) treating the cells with an agent that generates a reactive oxygen species in the cells, and
- c) evaluating survival of the cells, wherein an increase in the expression of an antioxidant enzyme, relative to the expression of the antioxidant enzyme within a population of cells that has not been exposed to the compound, indicates that the compound attenuates the protection an estrogen confers on a population of cells.
A method for determining whether a compound effects the protection an estrogen confers on a population of cells, the method comprising
- a) exposing the cells to the compound and the estrogen,
- b) exposing the cells with an agent that generates a reactive oxygen species in the cells, and
- c) evaluating the expression of an antioxidant enzyme in the cells, wherein a change in the expression level of the antioxidant enzyme, relative to that within a population of cells that has not been exposed to estrogen and the compound, indicates that the compound effects the protection an estrogen confers on a population of cells.
Dependencies are inferred from the claim text.