Combinatorial screening of mixed populations of organisms
AssigneeDiversa Corporation
InventorJay M. Short
Life of this patent
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Abstract
Provided is a method of screening gene libraries derived from a mixed population of organisms for a bioactivity or biomolecule of interest. The mixed population of organisms can be a cultured population or an uncultured population from, for example, the environment. Also provided are methods of screening isolates or enriched populations of organisms, which isolates include a population that is spatially, temporally, or hierarchical, for example, of a particular species, genus, family, or class of organisms. Identified clones containing a biomolecule or bioactivity of interest can be further variegated or the DNA contained in the clone can be variegated to create novel biomolecules or bioactivities of interest.
Claims20
A method of discovering bioactivity or a biomolecule of interest comprising:
- collecting samples containing organisms from a diverse set of environments;
- recovering nucleic acid molecules from the organisms;
- obtaining one or more proteins from the recovered nucleic acid molecules;
- screening one or more of the obtained proteins for a bioactivity or biomolecule of interest;
- if a screened protein exhibits the bioactivity or biomolecule of interest but does not exhibit a desired characteristic, employing one or more evolution technologies to attempt to cause the protein to exhibit the desired characteristic.
A method of discovering bioactivity or a biomolecule of interest comprising:
- collecting samples containing organisms from a diverse set of environments;
- identifying one or more of the organisms as having originated from environments having characteristics consistent with a first desired characteristic;
- screening one or more proteins obtained from the identified organisms for a bioactivity or biomolecule of interest;
- if a screened protein exhibits the bioactivity or biomolecule of interest but does not exhibit a second desired characteristic, employing one or more evolution technologies to attempt to cause the protein to exhibit the second desired characteristic.
A method of discovering bioactivity or a biomolecule of interest from a collection of environmental samples containing organisms from a diverse set of environments, the method comprising:
- recovering nucleic acid molecules from the organisms;
- obtaining one or more proteins from the recovered nucleic acid molecules;
- screening one or more obtained proteins for a bioactivity or biomolecule of interest;
- if a screened protein exhibits the bioactivity or biomolecule of interest but does not exhibit a first desired characteristic, employing one or more evolution technologies to attempt to cause the protein to exhibit the first desired characteristic.
A method of discovering bioactivity or a biomolecule of interest from a collection of environmental samples containing organisms from a diverse set of environments wherein nucleic acid molecules have been recovered from the organisms and one or more proteins have been obtained from the recovered nucleic acid molecules, the method comprising:
- screening one or more of the obtained proteins for a bioactivity or biomolecule of interest;
- if a screened protein exhibits the bioactivity or biomolecule of interest but does not exhibit a first desired characteristic, employing one or more evolution technologies to attempt to cause the protein to exhibit the first desired characteristic.
Dependencies are inferred from the claim text.