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US 2002/0164575 A1Published application
Gene identification
AssigneeSangamo BioSciences, Inc., a Delaware Corporation
InventorsCasey C. Case, Fyodor Urnov
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Abstract
The present disclosure provides methods and compositions for identifying a particular genomic sequence as a gene and/or a coding region, once that sequence has been tentatively identified as a gene based on genomic analysis using one or more gene prediction algorithms. The methods include the use of exogenous molecules such as zinc finger proteins which are capable of binding to and modulating expression of gene transcription, targeted to putative gene sequences, followed by assay for one or more selected phenotypes.
Claims30
A method for identifying a gene;
- wherein the method comprises:
- (a) obtaining a putative gene sequence (PGS);
- (b) contacting a cell with a zinc finger protein, wherein the cell comprises the putative gene sequence, and wherein the zinc finger protein binds to and modulates expression of the putative gene sequence;
- and
- (c) assaying the cell for at least one selected phenotype;
- wherein, if one or more of the selected phenotypes are observed, the putative gene sequence is identified as a gene.
3 more elements
Dependencies are inferred from the claim text.