High molecular weight surface proteins of non-typeable haemphilus
OwnerStephen J. Barenkampinventor
Life of this patent
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Abstract
High molecular weight surface proteins of non-typeable Haemophilus influenzae which exhibit immunogenic properties and genes encoding the same are described. Specifically, genes coding for two immunodominant high molecular weight proteins, HMW1 and HMW2, have been cloned, expressed and sequenced, while genes coding for high molecular proteins HMW3 and HMW4 have also been cloned, expressed and sequenced.
Claims13
An isolated and purified nucleic acid molecule encoding a high molecular weight protein (HMW) HMW3 or HMW4 of a non-typeable Haemophilus strain or a variant or fragment of said protein retaining the immunological ability to protect against disease caused by a non-typeable Haemophilus strain, having:
- (a) the DNA sequence shown in FIG. 8 (SEQ ID No:
- 7) and encoding protein HMW-3 having the derived amino acid sequence of FIG. 10 (SEQ ID No:
- 9), or
- (b) the DNA sequence shown in FIG. 9 (SEQ ID No:
- 8) and encoding protein HMW4 having the derived amino acid sequence of FIG. 10 (SEQ ID No:
- 10).
An isolated and purified nucleic acid molecule encoding a high molecular weight protein (HMW) of a non-typeable Haemophilus strain, which is selected from the group consisting of:
- (a) a DNA sequence as shown in any one of FIGS. 8 and 9 (SEQ ID Nos:
- 7 and 8);
- (b) a DNA sequence encoding an amino acid sequence as shown in FIG. 10 (SEQ ID Nos:
- 9 and 10);
- or
- (c) a DNA sequence encoding a high molecular weight protein of a non-typeable Haemophilus strain which hybridizes under stringent conditions to any one of the DNA sequences of (a) and (b).
An isolated and purified high molecular weight (HMW) protein of non-typeable Haemophilus or any variant or fragment thereof retaining the immunological ability to protect against disease caused by a non-typeable Haemophilus strain, which is characterized by at least one surface-exposed B-cell epitope which is recognized by monoclonal antibody AD6.
A synthetic peptide having an amino acid sequence containing at least six amino acids and no more than 150 amino acids and corresponding to at least one protective epitope of a high molecular weight protein HMW1, HMW2, HMW3 or HMW4 of non-typeable Haemophilus influenzae, wherein the epitope is recognized by at least one of monoclonal antibodies AD6 and 10C5.
Dependencies are inferred from the claim text.