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OwnerFernando J.R. do Couto, Roberto L. Ceriani +1inventors
InventorsFernando J.R. do Couto, Roberto L. Ceriani, Jerry A. Peterson
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The present invention provides recombinant peptides that specifically and selectively bind to the human milk fat globule (HMFG) antigen, BA46. In particular, the present invention provides recombinant variants of the Mc3 antibody, including humanized versions of Mc3. The variant Mc3 peptides are particularly useful for diagnostic, prognostic, and therapeutic applications in the field of breast cancer. The present invention also provides methods for the humanization of antibodies such as murine monoclonal antibodies. The novel humanization methods are applied to the production of humanized Mc3 antibodies and it is shown that these humanized antibodies retain the ability to engage in high affinity binding to their cognate antigen. Such humanization enables the use of these antibodies for immunodiagnostic and immunotherapeutic applications in humans.
A recombinant Mc3 antibody which binds to BA46 antigen of the human milk fat globule (HMFG), said antibody comprising at least one modified variable region, said modified variable region selected from the group consisting of:
A method of humanizing a non-human antibody comprising replacing one or more framework amino acid residues in a variable region of said antibody with corresponding framework amino acids from a human variable region wherein important non-human framework residues, as defined by the buried-residue-modification technique, are retained in their original form.
A method of humanizing a non-human antibody comprising replacing one or more framework amino acid residues in a variable region of said antibody with corresponding framework amino acids from a human variable region consensus sequence wherein important non-human framework residues, as defined by the buried-residue-modification technique, are retained in their original form.
Dependencies are inferred from the claim text.