US 2002/0164327 A1 · Pharmaceutical composition of F(ab)2 antibody fragments and a process for the preparation thereof

Abstract

The present invention is directed to a pharmaceutical composition comprising F(ab)2 antibody fragments that are preferably free from albumin and of whole antibodies and also substantially free of pyrogens, and an effective amount of a pharmaceutically acceptable carrier. It is also directed to a method for the production of pharmaceutical composition comprising (F(ab)2 antibody fragments using serum or blood plasma of a mammal that has been previously immunized, as a source of antibodies. The serum or blood plasma is digested with an enzyme pepsin, followed by separation and purification until the pharmaceutical composition of F(ab)2 fragments is free of albumin and complete antibodies, and substantially free of pyrogens.

Claims29

Dependent claims

A method for the preparation of F(ab)2 antibody fragments comprising the following steps:

a) obtaining blood plasma from immunized mammals in septic conditions;
b) contacting the plasma obtained with sterile pepsin in order to digest the antibodies;
c) removing the albumin, fibrinogen and other undesirable substances present in the plasma or its digestion products;
d) recovering the resulting F(ab)2 fragments from the solution;
and
e) optionally, purifying the F(ab)2 fragments thus obtained.

3 more elements

Dependencies are inferred from the claim text.

Pharmaceutical composition of F(ab)2 antibody fragments and a process for the preparation thereof

Life of this patent

Pharmaceutical composition of F(ab)2 antibody fragments and a process for the preparation thereof

Life of this patent

Abstract

Claims29