Back to results
US 2002/0164313 A1Published application
Compositions comprising preconditioned myoblasts having enhanced fusion properties
OwnerJacques P. Tremblayinventor
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerJacques P. Tremblayinventor
Estimating the term…
The present invention relates to a method for preconditioning healthy donor's myoblasts in vitro before transplantation thereof in compatible patients suffering of recessive myopathies, particularly of muscular dystrophy. This in vitro preconditioning improves the success of the transplantation while not requiring an in vivo preconditioning of the patient's muscle by irradiation or by administering muscular toxin. The invention further relates to compositions comprising such preconditioned myoblasts. The preconditioning comprises pre-treating the transplanted myoblasts with human fibroblast growth factor (bFGF). The transplantation is made with the whole culture along with bFGF. A concentration of 100 ng/ml bFGF improved the myoblasts fusion by a four fold average.
A composition comprising a culture of myoblasts to be transplanted into a recipient muscle tissue, together with a suitable pharmaceutical carrier, said culture comprising myoblasts and a muscle-fusion promoting amount of human basic fibroblast growth factor (bFGF), which transplanted myoblasts have been grown in the presence of said amount of bFGF prior to transplantation into said recipient muscle tissue, said amount being capable of increasing by at least two fold the fusion between transplanted and recipient myoblasts over and above the fusion of a same number of transplanted myoblasts not grown in the presence of said promoting amount of bFGF.
A method of improving the fusion of myoblasts upon transplantation thereof into a recipient muscular tissue, comprising the steps of:
A method of improving the fusion of myoblasts upon transplantation thereof into a recipient muscular tissue comprising the steps of:
Dependencies are inferred from the claim text.