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OwnerJoel F. Habener, Henryk Zulewski +4inventors
InventorsJoel F. Habener, Henryk Zulewski, Melissa K. Thomas, Elizabeth J. Abraham, Mario Vallejo, Colin A. Leech
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Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin and GLP-1 receptor have been identified as molecular markers for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby stem cells which express one or both of nestin and GLP-1R can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.
A method of treating a patient with diabetes mellitus, comprising the steps of:
A method of treating a patient with diabetes mellitus, comprising the steps of:
A method of treating a patient with diabetes mellitus, comprising the steps of:
A method of treating a patient with diabetes mellitus, comprising the steps of:
A method of treating a patient with diabetes mellitus, comprising the steps of:
A method of treating a patient with diabetes mellitus, comprising the steps of:
A method of isolating a stem cell from a pancreatic islet of Langerhans, comprising the steps of:
A method of isolating a stem cell from a pancreatic islet of Langerhans, comprising the steps of:
A method of inducing the differentiation of a nestin-positive pancreatic stem cell into a pancreatic progenitor cell, comprising the step of:
A method of inducing the differentiation of a nestin-positive pancreatic stem cell into a pancreatic progenitor cell, comprising the step of:
An isolated, nestin-positive human pancreatic or liver stem cell that is not a neural stem cell.
An isolated, GLP-1R-positive human pancreatic stem cell that is not a neural stem cell.
A method of identifying a pancreatic cell as a stem cell, comprising the step of:
A method of identifying a pancreatic cell as a stem cell, comprising the step of:
A method of inducing a nestin-positive pancreatic stem cell to differentiate into hepatocytes, comprising the step of:
A method of inducing a GLP-1R-positive pancreatic stem cell to differentiate into hepatocytes, comprising the step of:
A method of treating a patient with liver disease, comprising the steps of:
A method of treating a patient with liver disease, comprising the steps of:
A method of treating a patient with liver disease, comprising the steps of:
A method of treating a patient with liver disease, comprising the steps of:
A method of treating a patient with liver disease, comprising the steps of:
A method of treating a patient with liver disease, comprising the steps of:
Dependencies are inferred from the claim text.