Capillary electrophoresis systems and additives
AssigneeSebia
InventorsGeorges Nouadje, Frederic Robert
Life of this patent
Estimating the term…
Abstract
Capillary electrophoresis systems, reagents, and processes for analyzing a sample which includes at least one protein constituent. The processes involve introducing the sample into a capillary tube containing a buffer system, wherein said buffer system includes at least one additive having an hydrophobic interaction with said at least one protein constituent and providing said at least one protein constituent with at least one negative charge thereby modifying the electrophoretic mobility
Claims33
An alkaline pH, free solution capillary electrophoresis process for analyzing a sample comprising at least one protein constituent, said method comprising:
- introducing the sample into a capillary tube containing a buffer system, wherein said buffer system comprises at least one additive having an hydrophobic interaction with said at least one protein constituent and providing said at least one protein constituent with at least one negative charge thereby modifying the electrophoretic mobility.
2 The method of claim 1, wherein said additive has a concentration in said buffer system in the range of 0.1 mM to 500 mM.
The method of claim 12, wherein said additive in said buffer system does not exceed the critical micellar concentration of said additive in said buffer.
A method for separating at least one protein constituent in a sample comprising a protein selected from albumin, α1-globulin, α2-globulin, β-globulin, β1-globulin, β2-globulin and γ-globulin, said method comprising passing said at least one protein constituent into a capillary containing a buffer system comprising at least one further additive having a hydrophobic interaction with albumin.
A method for electrophoretic separation, by alkaline pH, free solution capillary electrophoresis, of protein constituents in a liquid sample, said method comprising passing said at least one protein constituent into a capillary containing a buffer system further comprising at least one additive, wherein said additive is a compound comprising an anionic pole with a pH of more than 9 and a hydrophobic portion.
An electrolyte composition for capillary electrophoresis, which comprises in a support at least one buffer and an additive that has a hydrophobic interaction with albumin.
A composition for capillary electrophoresis, which comprises at least one buffer system and an additive comprising a hydrophobic portion composed of at least one linear or non linear alkyl chain containing 4 to 22 carbon atoms, and/or at least a combination or 1 to 10 cyclic aromatic groups or cyclic non-aromatic groups, and an anionic pole comprising at least one group selected from sulphonates, carboxylates, sulphates, phosphates and carbonates.
Dependencies are inferred from the claim text.