Sugarcane fractioning system
AssigneesHoniron Corporation (a Louisiana Corporation), The Texas A&M University (an Agency of the State of Texas)
InventorsT. Erik Mirkov, Jean P. Monclin, Adam Barrilleaux, James E. Irvine, Francis Moonan
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Abstract
A method of extracting and purifying recombinant protein(s) from transgenic sugarcane is disclosed. Fractioning of sugarcane juice that has been extracted from the cane stalks is obtained by using a multiple stage filtering process that uses multiple stages of decreasing porosity (preferably screening) followed by preferably membrane type filters, ion exchange, membrane adsorber, and chromatographic processes.
Claims13
A method of refining sugarcane to extract protein matter comprising the steps of:
- a) providing a sugarcane feedstock to be processed;
- b) extracting the sugarcane feedstock to extract cane pression juice;
- c) cleaning the pression sugarcane juice to remove particulate matter;
- d) adjusting the pH of the sugarcane juice to a pH of at least 4.5;
- e) transmitting the juice from steps “a” through “d” to a membrane separation system in order to produce two apt fractions, one of the fractions containing a protein of interest.
The method of claim 12 wherein there are a plurality of fraction tanks and each permeate fraction is transmitted to a tank.
A process for extraction of protein from sugarcane, comprising the steps of:
- a) extracting sugarcane juice from a feedstock of protein contained in sugar cane stalks;
- b) preliminarily screening said juice to remove particulate matter, using a plurality of screens spanning a range of porosity of between 50 and 500 microns porosity;
- c) treating the cane juice with multiple stages of ultra-clarifying filtration of decreasing porosity, including some ultra-clarifying filtration that includes membrane separation.
Dependencies are inferred from the claim text.