Genes and methods for manipulation of growth
AssigneePioneer Hi-Bred International, Inc.
InventorsGurmukh S. Johal, Dilbag S. Multani, Steven P. Briggs
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Abstract
The invention relates to the genetic manipulation of organisms, particularly to the expression of P-glycoprotein genes in transformed organisms, preferably plants. Nucleotide sequences for the P-glycoprotein genes, particularly the Br2 gene of maize, and methods for their use are provided. The sequences find use in modifying the growth of organisms, particularly plants.
Claims22
An isolated nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising the Br2 gene of maize;
- (b) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (c) a nucleotide sequence set forth in SEQ ID NO:
- 2;
- (d) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1;
- (e) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 2;
- (f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:3;
- (g) a nucleotide sequence encoding at least 58 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (h) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 1.
- (i) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 2.
- (j) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(i);
- and
- (k) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequence of (b) or (c), or to the complementary sequence of (b) or (c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant growth or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
A transformed plant having stably incorporated into its genome a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence comprising the Br2 gene of maize;
- (b) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (c) a nucleotide sequence set forth in SEQ ID NO:
- 2;
- (d) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1;
- (e) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 2;
- (f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:3;
- (g) a nucleotide sequence encoding at least 58 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (h) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 1.
- (i) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 2.
- (j) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(i);
- and
- (k) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequence of (b) or (c), or to the complementary sequence of (b) or (c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant growth or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
A method for modifying the growth of a plant, said method comprising transforming a plant with a nucleotide sequence encoding a P-glycoprotein, wherein said P-glycoprotein finctions to control growth of a plant, said nucleotide sequence operably linked to a promoter capable of driving the expression of said sequence in said plant and said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence comprising the Br2 gene of maize;
- (b) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (c) a nucleotide sequence set forth in SEQ ID NO:
- 2;
- (d) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1;
- (e) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 2;
- (f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:3;
- (g) a nucleotide sequence encoding at least 58 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (h) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 1.
- (i) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 2.
- (j) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(i);
- and
- (k) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequence of (b) or (c), or to the complementary sequence of (b) or (c).
A transformed plant cell having stably incorporated into its genome a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleotide sequence comprising the Br2 gene of maize;
- (b) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (c) a nucleotide sequence set forth in SEQ ID NO:
- 2;
- (d) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1;
- (e) a nucleotide sequence consisting of at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 2;
- (f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:3;
- (g) a nucleotide sequence encoding at least 58 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (h) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 1.
- (i) a nucleotide sequence comprising at least 70% identity to the sequence set forth in SEQ ID NO:
- 2.
- (j) a nucleotide sequence that is complementary to the nucleotide sequence of any one of (a)-(i);
- and
- (k) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequence of (b) or (c), or to the complementary sequence of (b) or (c);
- wherein said nucleotide sequence encodes a P-glycoprotein that controls plant growth or said nucleotide sequence is complementary to a nucleotide sequence that encodes said P-glycoprotein.
Dependencies are inferred from the claim text.