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US 2002/0162141 A1Published application
Transgenic plant protein expression and recovery system
OwnerT. Erik Mirkov, James E. Irvineinventors
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Abstract
A protein expression and recovery system in which transgenes are expressed in target plant varieties. Transformation is accomplished through bombardment of embryonic calli with plasmid-coated tungsten particles. Transformed calli are grown on tissue culture medium to produce transgenic plants which are then screened for presence of the transgene DNA or the polypeptide of interest. Transgenic plants are then cultivated. Cultivated plants may be harvested for extraction of the polypeptide.
Claims20
A method of producing a polypeptide comprising:
- selecting a target plant variety;
- selecting a transgene DNA molecule encoding the polypeptide;
- constructing a plasmid comprising a ubiquitin promoter and a nopaline synthase transcriptional terminator operably linked to the transgene DNA molecule;
- transforming an embryonic calli of the target plant variety with tungsten particles coated with the plasmid;
- culturing the bombarded calli on a tissue culture medium to regenerate transgenic plants;
- cultivating the transgenic plants.
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