Primordial germ cell-based germ line production of birds
OwnerAlexander Baguisi, Karl M. Ebertinventors
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Abstract
The invention features an isolated avian gonadal cell, e.g., an ovarian cell or a testes cell, containing a heterologous nucleic acid as well as a method of introducing a nucleic acid molecule into the genome of an avian species, by contacting a population of isolated gonadal cells derived from a chick embryo with the nucleic acid molecule to yield transfected gonadal cells, and transferring the transfected gonadal cells to a fertilized avian egg
Claims25
An isolated avian gonadal cell comprising a heterologous nucleic acid.
A method of introducing a nucleic acid molecule into the genome of an avian species, comprising contacting a population of isolated gonadal cells obtained from a chick embryo with said nucleic acid molecule to yield transfected gonadal cells, and transferring said transfected gonadal cells to a fertilized recipient avian egg.
An isolated avian gonadal cell, comprising a genetic disruption of an endogenous gene, wherein said disruption inhibits production of a functional gene product.
An avian egg comprising a xenogeneic primordial germ cell.
Dependencies are inferred from the claim text.