Transgenic mice containing mGluR8 metabotropic glutamate receptor gene disruptions
OwnerKeith D. Alleninventor
Life of this patent
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Abstract
The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in an mGluR8 gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions.
Claims25
A targeting construct comprising:
- (a) a first polynucleotide sequence homologous to an mGluR8 gene;
- (b) a second polynucleotide sequence homologous to the mGluR8 gene;
- and
- (c) a selectable marker.
A method of producing a targeting construct, the method comprising:
- (a) providing a first polynucleotide sequence homologous to an mGluR8 gene;
- (b) providing a second polynucleotide sequence homologous to the mGluR8 gene;
- (c) providing a selectable marker;
- and
- (d) inserting the first sequence, second sequence, and selectable marker into a vector, to produce the targeting construct.
A method of producing a targeting construct, the method comprising:
- (a) providing a polynucleotide comprising a first sequence homologous to a first region of an mGluR8 gene and a second sequence homologous to a second region of an mGluR8 gene;
- and
- (b) inserting a positive selection marker between the first and second sequences to form the targeting construct.
A cell comprising a disruption in an mGluR8 gene.
A non-human transgenic animal comprising a disruption in an mGluR8 gene.
A method of identifying an agent that modulates the expression of an mGluR8, the method comprising:
- (a) providing a non-human transgenic animal comprising a disruption in an mGluR8 gene;
- (b) administering an agent to the non-human transgenic animal;
- and
- (c) determining whether the expression of mGluR8 in the non-human transgenic animal is modulated.
A method of identifying an agent that modulates the function of an mGluR8, the method comprising:
- (a) providing a non-human transgenic animal comprising a disruption in an mGluR8 gene;
- (b) administering an agent to the non-human transgenic animal;
- and
- (c) determining whether the function of the disrupted mGluR8 gene in the non-human transgenic animal is modulated.
A method of identifying an agent that modulates the expression of mGluR8, the method comprising:
- (a) providing a cell comprising a disruption in an mGluR8 gene;
- (b) contacting the cell with an agent;
- and
- (c) determining whether expression of the mGluR8 is modulated.
A method of identifying an agent that modulates the function of an mGluR8 gene, the method comprising:
- (a) providing a cell comprising a disruption in an mGluR8 gene;
- (b) contacting the cell with an agent;
- and
- (c) determining whether the function of the mGluR8 gene is modulated.
A transgenic mouse comprising a homozygous disruption in a gene comprising SEQ ID NO:
- 1, or a homolog thereof.
Dependencies are inferred from the claim text.