High throughput genetic screening of lipid and cholesterol processing using fluorescent compounds
OwnerSteven Farber, Michael Packinventors
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Abstract
The present invention utilizes fluorescent lipids, particularly quenched phospholipid or cholesterol analogues, to facilitate screening for phenotypes representing perturbations of lipid processing; screening for genetic mutations that lead to disorders of phospholipid and/or cholesterol metabolism; and screening of compounds designed to treat disorders of phospholipid and/or cholesterol metabolism. The present invention is a mutant fish containing a metabolic defect affecting phospholipid and cholesterol processing. The mutant fish is useful for studying phospholipid and cholesterol metabolism and for screening compounds designed to treat disorders of phospholipid and/or cholesterol metabolism.
Claims14
A mutant fish whose genome comprises a homozygous disruption in a metabolic pathway of cholesterol and/or lipid processing, wherein said disruption reduces cholesterol absorption in a gall bladder of said mutant fish and wherein said mutant fish are phenotypically normal.
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