Non-enzymatic large scale synthesis of RNA
OwnerAnastassia Kanavarioti, David W. Deamer +2inventors
InventorsAnastassia Kanavarioti, David W. Deamer, Pierre-Alain M. Monnard, Claude F. Bernasconi
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Abstract
This invention pertains to the development of methods that accomplish efficient, non-enzymatic, nucleic acid-directed (e.g. RNA-directed) nucleic acid (e.g. RNA) synthesis. In certain embodiments the methods provide conditions that favor oligouridylate synthesis with excellent yield and, at least, up to 30% regioselectivity favoring the RNA linkage. The methods preferably involve contacting, in the presence of lead ions (Pb2+) and/or tin ions (Sn2+) and, optionally, magnesium ions (Mg2+), a template nucleic acid with a nucleotide derivatized with an imidazolide.
Claims72
A method of synthesizing a ribonucleic acid, said method comprising contacting a template nucleic acid, in the presence of lead ions (Pb2+) or tin (Sn2+) ions, with a nucleotide derivatized with an imidazolide.
A method of synthesizing a ribonucleic acid, said method comprising contacting, in the presence of lead ions (Pb2+) or tin (Sn2+) ions, a plurality of activated nucleotides wherein said activated nucleotides are nucleotides derivatized with an imidazolide.
A kit for the synthesis of a ribonucleic acid, said kit comprising:
- a container containing a nucleotide derivatized with an imidazolide;
- and
- a container containing a lead salt or a tin salt.
Dependencies are inferred from the claim text.