Compositions inducing cleavage of RNA motifs

A composition that induces cleavage of an RNA substrate, the composition comprising:

• 5′-Z1-Z2-Z3-3′
• wherein Z1 and Z3 are oligomeric sequences which (1) are comprised of nucleotides, nucleotide analogues, or both, or (2) are oligonuleotide analogues, wherein the oligomeric sequences specifically interact with the RNA substrate by hybridization.
• wherein Z2 consists of
• 5′-X12X13X14X15.1-3′, or5′-X12X13X14X15.1-3′,
• wherein Z2 is comprised of nucleotides, nucleotide analogues, or both, wherein the nucleotides and nucleotide analogues each have the structure
• 3
• wherein each B is independently adenin-9-yl, cytosin-1-yl, guanin-9-yl, uracil-1-yl, uracil-5-yl, hypoxanthin-9-yl, thymin-1-yl, 5-methylcytosin-1-yl, 2,6-diaminopurin-9-yl, purin-9-yl, 7-deazaadenin-9-yl, 7-deazaguanin-9-yl, 5-propynylcytosin-1-yl, 5-propynyluracil-1-yl, isoguanin-9-yl, 2-aminopurin-9-yl, 6-methyluracil-1-yl, 4-thiouracil-1-yl, 2-pyrimidone-1-yl, quinazoline-2,4-dione-1-yl, xanthin-9-yl, N2-dimethylguanin-9yl or a functional equivalent thereof,
• wherein each V is independently an O, S, NH, or CH2 group,
• wherein each W is independently selected from the group consisting of —H, —OH, —COOH, —CONH2, —CONHR1, —CONR1R2, —NH2, —NHR1, —NR1R2, —NHCOR1, —SH, SR1, —F, —ONH2, —ONHR1, —ONR1R2, —NHOH, —NHOR1, —NR2OH, —NR2OR1, substituted or unsubstituted C1-C10 straight chain or branched alkyl, substituted or unsubstituted C2-C10 straight chain or branched alkenyl, substituted or unsubstituted C2-C10 straight chain or branched alkynyl, substituted or unsubstituted C1-C10 straight chain or branched alkoxy, substituted or unsubstituted C2-C10 straight chain or branched alkenyloxy, and substituted or unsubstituted C2-C10 straight chain or branched alkynyloxy, wherein the substituents are independently halogen, cyano, amino, carboxy, ester, ether, carboxamide, hydroxy, or mercapto, wherein R1 and R2 are, independently, substituted or unsubstituted alkyl, alkenyl, or alkynyl groups, where the substituents are independently halogen, cyano, amino, carboxy, ester, ether, carboxamide, hydroxy, or mercapto,
• wherein D and E are residues which together form a phosphodiester or phosphorothioate diester bond between adjacent nucleosides or nucleoside analogues or together form an analogue of an internucleosidic bond,
• wherein in X15.1, B is hypoxanthin-9-yl or a functional equivalent thereof,
• wherein in X12, B is independently guanin-9-yl, hypoxanthin-9-yl or 7-deazaguanin-9-yl;
• wherein in X13 and X14, B is independently adenin-9-yl, 2,6-diaminopurin-9-yl, purin-9-yl or 7-deazaadenin-9-yl;
• wherein in Xˆ 12, B is independently adenin-9-yl, cytosin-1-yl, guanin-9-yl, uracil-1-yl, uracil-5-yl, hypoxanthin-9-yl, thymin-1-yl, 5-methylcytosin-1-yl, 2,6-diaminopurin-9-yl, purin-9-yl, 7-deazaadenin-9-yl, 7-deazaguanin-9-yl, 5-propynylcytosin-1-yl, 5-propynyluracil-1-yl, isoguanin-9-yl, 2-aminopurin-9-yl, 6-methyluracil-1-yl, 4-thiouracil-1-yl, 2-pyrimidone-1-yl, quinazoline-2,4-dione-1-yl, xanthin-9-yl, N2-dimethylguanin-9-yl or a functional equivalent thereof.