ErbB4 receptor-specific neuregulin related ligands and uses therefor
AssigneeGenentech, Inc.
InventorsPaul J. Godowski, Melanie Rose Mark, Dong Xiao Zhang
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Abstract
The invention concerns a novel neuregulin related ligand (NRG3) including fragments and variants thereof, as new members of the neuregulin family of compounds. The invention also concerns methods and means for producing NRG3. The native polypeptides of the invention are characterized by containing an extracellular domain including an EGF-like domain, a transmembrane domain and a cytoplasmic domain. Isolated nucleotide sequences encoding such polypeptides, expression vectors containing the nucleotide sequences, recombinant host cells transformed with the vectors, and methods for the recombinant production for the novel NRG3s are also within the scope of the invention.
Claims38
A polypeptide comprising an amino acid sequence encoding an EGF-like domain, wherein the amino acid sequence has the binding characteristics of NRG3.
A polypeptide that binds ErbB4 receptor, which polypeptide is selected from the group consisting of
- (a) a polypeptide comprising an amino acid sequence having at least 75% sequence homology to the extracellular domain NRG3 (SEQ ID NO:3 or 7);
- (b) a polypeptide comprising an amino acid sequence having at least 75% sequence homology to SEQ ID NO:2 or SEQ ID NO:6;
- (c) a further mammalian homologue of polypeptide (a) or (b):
- (d) a soluble form of any of the polypeptides (a)-(c) having a transmembrane domain that cannot anchor the polypeptide in a cell membrane;
- and
- (e) a derivative of any of the polypeptides (a)-(d) having the binding characteristics of NRG3.
Dependencies are inferred from the claim text.