Antibodies specific for hlh beta core fragment and uses thereof
AssigneeThe Trustees of Columbia University in the City of New York
InventorsROBERT E. CANFIELD, STEVEN BIRKEN, JOHN O'CONNOR, GALINA KOVALEVSKAYA
Life of this patent
Estimating the term…
Abstract
This invention provides an antibody which specifically binds to hLHβcf without cross-reacting with hLH, hLHβ or hCGβcf. In an embodiment, the monoclonal antibody is designated B505. In a further embodiment, the hybridoma cell line producing the monoclonal antibody B 505 is designated ATCC Accession No. 12000. This invention provides different uses of the antibodies. Finally, this invention provides a method for determining the amount of hLHβcf or hLHβcf-related molecule in a sample.
Claims26
An antibody which specifically binds to hLHβcf without cross-reacting with hLH, hLHβ or hCGβcf.
A method for determining the amount of hLHβcf or hLHβcf-related molecule in a sample comprising steps of:
- (a) contacting the sample with an antibody which specifically binds to hLHβcf without cross-reacting with hLH, hLHβ or hCGβcf under condition permitting formation of a complex between the antibody and hLHβcf;
- and
- (b) determining the amount of complexes formed, thereby determining the amount of hLHβcf or hLHβcf-related molecule in the sample.
A method for determining the amount of hLHβcf or hLHβcf-related molecule in a sample comprising steps of:
- (a) contacting at least one capturing antibody selected from a group consisting of B503, B504 and B509 with a solid matrix under conditions permitting binding of capturing antibody with the solid matrix;
- (b) contacting the bound matrix with the sample under conditions permitting binding of the antigen present in the sample with the capturing antibody;
- (c) separating the bound matrix and the sample;
- (d) contacting the separated bound matrix with an antibody which specifically binds to hLHβcf without cross reacting with hLH, hLHβ or hCGβcf under conditions permitting binding of antibody and antigen in the sample;
- and
- (e) determining the amount of bound antibody on the bound matrix, thereby determining the amount of hLHβcf or hLHβcf-related molecule in the sample.
A method for determining the amount of hLHβcf or hLHβcf-related molecule in a sample comprising steps of:
- (a) contacting a capturing antibody which specifically binds to hLHβcf without cross-reacting with hLH, hLHβ or hCGβcf with a solid matrix under conditions permitting binding of the antibody with the solid matrix;
- (b) contacting the bound matrix with the sample under conditions permitting binding of the antigen present in the sample with the bound capturing antibody;
- (c) separating the bound matrix and the sample;
- (d) contacting the separated bound matrix with at least one detecting antibody selected from a group consisting of B503, B504 and B509 under conditions permitting binding of antibody and antigen in the sample;
- and
- (e) determining the amount of bound antibody on the bound matrix, thereby determining the amount of hLHβcf or hLHβcf-related molecule in the sample.
A method of detecting ovulation in a female subject comprising:
- (a) obtaining samples from the female subject;
- and
- (b) determining the amount of hLHβcf or hLHβcf-related molecule in the samples, the presence of a peak of hLHβcf indicating the occurrence of ovulation.
A method for reducing the amount of hLHβcf or hLHβcf-related molecule in a sample comprising steps of:
- (a) contacting the sample with an antibody which specifically binds to hLHβcf without cross-reacting with hLH, hLHβ or hCGβcf under conditions permitting formation of complex between the antibody and hLHβcf;
- and
- (b) removing the complex formed, thereby the amount of hLHβcf or hLHβcf-related molecule in the sample.
Dependencies are inferred from the claim text.