Novel DKR polypeptides
AssigneeAmgen Inc.
InventorsMichael Brian Bass, John Kevin Sullivan, Lars Eyde Theill, Daguang Wang
Life of this patent
Estimating the term…
Abstract
Disclosed are nucleic acid molecules encoding novel DKR polypeptides. Also disclosed are methods of preparing the nucleic acid molecules and polypeptides, and methods of using these molecules.
Claims20
An isolated nucleic acid molecule encoding a biologically active DKR polypeptide selected from the group consisting of:
- (a) the nucleic acid molecule comprising SEQ ID NO:1;
- (b) the nucleic acid molecule comprising SEQ ID NO:2;
- (c) the nucleic acid molecule comprising SEQ ID NO:3;
- (d) the nucleic acid molecule comprising SEQ ID NO:4;
- (e) the nucleic acid molecule comprising SEQ ID NO:5;
- (f) the nucleic acid molecule comprising SEQ ID NO:6;
- (g) the nucleic acid molecule comprising SEQ ID NO:7;
- (h) the nucleic acid molecule comprising SEQ ID NO:75;
- (i) the nucleic acid molecule comprising SEQ ID NO:76;
- (j) the nucleic acid molecule comprising SEQ ID NO:77;
- (k) the nucleic acid molecule comprising SEQ ID NO:78;
- (l) the nucleic acid molecule encoding the polypeptide of SEQ ID NO:8;
- (m) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:9;
- (n) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:10, or a biologically active fragment thereof;
- (o) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:11, or a biologically active fragment thereof;
- (p) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:12, or a biologically active fragment thereof;
- (q) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:13, or a biologically active fragment thereof;
- (r) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:14, or a biologically active fragment thereof
- (s) a nucleic acid molecule that encodes a polypeptide that is at least 85 percent identical to the polypeptide of SEQ ID NOs:
- 10, 11, 12, 13, or 14;
- (t) a nucleic acid molecule that encodes a biologically active DKR polypeptide that has 1-100 amino acid substitutions and/or deletions as compared with the polypeptide of any of SEQ ID NOs:8, 9, 10, 11, 12, 13, or 14;
- and
- (u) a nucleic acid molecule that hybridizes under conditions of high stringency to any of (c), (d), (e), (f), (g), (h), (i), (k), (l), (m), (n), (o), (p), (q), (r), (s), and (t) above.
2 An isolated nucleic acid molecule that is the complement of the nucleic acid molecule of claim 1.
An isolated nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7.
An isolated nucleic acid molecule encoding the polypeptide of SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13,or SEQ ID NO:14.
An isolated nucleic acid molecule encoding a biologically active DKR polypeptide selected from the group consisting of:
- amino acids 16-350, 21-350, 22-350, 23-350, 33-350, or 42-350, 21-145, 40-145, 40-150, 45-145, 45-145, 145-290, 145-300, 145-350, 150-290, 300-350, or 310-350 of SEQ ID NO:9;
- amino acids 15-266, 24-266, or 32-266 of SEQ ID NO:10;
- amino acids 17-259, 26-259, or 34-359 of SEQ ID NO:12;
- and amino acids 19-224, 20-224, 21-224, or 22-224 of SEQ ID NO:14.
A vector comprising the nucleic acid molecule of claim 2.
A biologically active DKR polypeptide selected from the group consisting of:
- (a) the polypeptide of SEQ ID NO:8;
- (b) the polypeptide of SEQ ID NO:9;
- (c) the polypeptide of SEQ ID NO:10;
- (d) the polypeptide of SEQ ID NO:11;
- (e) the polypeptide of SEQ ID NO:12;
- (f) the polypeptide of SEQ ID NO:13;
- (g) the polypeptide of SEQ ID NO:14;
- (h) a polypeptide that has 1-100 amino acid substitutions or deletions as compared with the polypeptide of any of (a)-(h) above;
- and
- (i) a polypeptide that is at least 85 percent identical to any of the polypeptides of (c)-(h) above.
A polypeptide selected from the group consisting of amino acids 16-350, 21-350, 22-350, 23-350, 33-350, 42-350, 21-145, 40-145, 40-150, 45-145, 45-145, 145-290, 145-300, 145-350, 150-290, 300-350, or 310-350 of SEQ ID NO:9;
- amino acids 15-266, 24-266, or 32-266 of SEQ ID NO:10;
- amino acids 17-259, 26-259, or 34-259 of SEQ ID NO:12;
- and amino acids 19-224, 20-224, 21-224, or 22-224 of SEQ ID NO:14.
Dependencies are inferred from the claim text.