Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
OwnerGyula Hadlaczky, Aladar A. Szalayinventors
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Abstract
Methods for preparing cell lines that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, methods for amplification of nucleic acids and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided are cell lines for use in the methods, and cell lines and chromosomes produced by the methods. Methods for use of the artificial chromosomes are also provided.6
Claims57
A method comprising:
- introducing a mammalian artificial chromosome that comprises nucleic acid encoding a therapeutic product into a cell;
- and
- introducing the resulting cell into a host animal;
- wherein the therapeutic product is expressed in the cell.
A method comprising:
- introducing a mammalian artificial chromosome that comprises nucleic acid encoding a therapeutic product into a cell of a host animal, wherein the therapeutic product is expressed in the cell.
A method comprising:
- introducing a mammalian artificial chromosome that comprises nucleic acid encoding a therapeutic product into tissue of a host animal, wherein the therapeutic product is expressed in cells of the tissue.
A method comprising:
- introducing a mammalian artificial chromosome that comprises nucleic acid encoding a gene product into a cell of a host animal, whereby the gene product is expressed in the cell.
Dependencies are inferred from the claim text.