Methods and compositions for producing a neurosalutary effect in a subject
AssigneeChildren's Medical Center Corporation
InventorLarry I. Benowitz
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Abstract
Methods and compositions for producing a neurosalutary effect in a subject are provided. These methods generally involve administering to a subject a therapeutically effective amount of a compound that modulates the activity of N-kinase, or analog thereof. Pharmaceutical and packaged formulations including the compounds of the invention, e.g., compounds that modulate the activity of N-kinase, are also provided.
Claims58
A method comprising administering to a subject a therapeutically effective amount of a compound that modulates the activity of N-kinase, thereby producing a neurosalutary effect in said subject.
A method comprising administering a therapeutically effective amount of a compound that modulates the activity of N-kinase to a subject suffering from a neurological disorder, thereby treating said subject suffering from a neurological disorder.
A method for identifying a compound capable of producing a neurosalutary effect in a subject, comprising contacting N-kinase, or a biologically active fragment thereof, with a test compound and determining the ability of the test compound to modulate the activity of N-kinase, thereby identifying a compound capable of producing a neurosalutary effect in a subject.
A method for identifying a compound capable of producing a neurosalutary effect in a subject, comprising
- contacting N-kinase or a biologically active fragment thereof, with a test compound, an N-kinase substrate, radioactive ATP, and Mn+2;
- and
- determining the ability of the test compound to modulate N-kinase dependent phosphorylation of the substrate, thereby identifying a compound capable of producing a neurosalutary effect in a subject.
An isolated N-kinase polypeptide of the type that:
- (a) is present in neonatal brain tissue;
- (b) is inhibited in the presence of 6-thioguanine;
- (c) is activated in the presence of Mn+2 but not by Mg+2 or Ca+2;
- (d) has a molecular weight of approximately 49 kDa;
- and
- (e) is eluted from a Cibacron Blue column at a NaCl concentration of 1.5-1.75 M.
An isolated nucleic acid molecule that encodes the polypeptide of SEQ ID NO:1.
Dependencies are inferred from the claim text.