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US 2002/0160537 A1Published application
Method of detecting cardiac ischemia using fatty acid binding protein
AssigneeTorrey Pines Institute for Molecular Studies
InventorAlan M. Kleinfeld
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Abstract
A method of detecting cardiac ischemia by detecting elevated levels of serum free fatty acids in serum unbound to serum albumin (FFAU) compared to an average FFAU level in individuals without cardiac ischemia, wherein the detection method uses a free fatty acid binding protein derivatized with a fluorescent moiety, is disclosed.
Claims14
A method of detecting cardiac ischemia in a mammal, comprising the steps of:
- providing a serum sample obtained from said mammal;
- mixing said serum sample with an aqueous solution and with a reagent comprising a fatty acid binding protein labeled with a fluorescent moiety, wherein said reagent exhibits a first fluorescence in an aqueous solution and a measurably different second fluorescence in an aqueous solution when said fatty acid binding protein is bound to a free fatty acid, wherein said free fatty acid is unbound to serum albumin;
- measuring said second fluorescence after said serum sample is mixed with said aqueous solution and said reagent to determine a concentration of said free fatty acid in said serum sample;
- and
- determining whether said concentration of said free fatty acid in said serum sample is indicative of cardiac ischemia.
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