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AssigneeUniversity of Medicine and Dentistry
InventorsOleg Mirochnitchenko, Jiang Wei, Masayori Inouye
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The present invention provides a novel gene, IAP, which encodes a soluble protein that is involved in the initiation of an inflammatory cytokine response and which may interact with the anti-inflammatory IL-13. The protein of the present invention may be involved as a central mediator in ischemia, reperfusion, asthma and other inflammation-induced pathological conditions. The invention relates to methods for using isolated polypeptides and polynucleotides, for detecting the early onset of chronic asthma, psoriasis, stroke, ischemia, reperfusion, leishmaniasis, helminthiasis, hypoxia, or other causes of renal, liver or heart failure in a mammal, where increased cytokine activity is known to play a role. The invention, further, relates to methods and compositions for detecting ischemia, specifically silent ischemia, by obtaining a test sample from a mammal, measuring the level of IAP mRNA or protein in the test sample, and determining if the level of IAP mRNA or protein measured in the test sample correlates with elevated levels indicative of an ischemia associated disease.
An isolated polynucleotide comprising a nucleotide sequence that has at least 70% identity to a nucleotide sequence encoding the IAP polypeptide of SEQ ID NO:2, said identity being over the entire region encoding SEQ ID NO:2.
An isolated polynucleotide, comprising a nucleotide sequence that has at least 70% identity to SEQ ID NO:1 or SEQ ID NO:3, said identity being calculated over the entire length of SEQ ID NO:1 or SEQ ID NO:3.
An expression vector comprising a polynucleotide, wherein said expression vector is capable of producing an IAP polypeptide comprising the amino acid sequence of SEQ ID NO:2 when said expression system is present in a compatible host cell.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide comprising a nucleotide sequence that has at least 70% identity to a nucleotide sequence encoding the IAP polypeptide of SEQ ID NO:5, said identity being over the entire region encoding for SEQ ID NO:5.
An isolated polynucleotide, comprising a nucleotide sequence that has at least 70% identity to SEQ ID NO:4, said identity being calculated over the entire length of SEQ ID NO:4.
An expression vector comprising a polynucleotide, wherein said expression vector is capable of producing an IAP polypeptide comprising the amino acid sequence of SEQ ID NO:5 when said expression system is present in a compatible host cell.
An isolated polypeptide, comprising the amino acid sequence of SEQ ID NO:5.
An isolated polynucleotide, comprising a nucleotide sequence that has at least 70% identity to a nucleotide sequence encoding the IAP polypeptide of SEQ ID NO:7, said identity being over the entire region encoding for SEQ ID NO:7.
An isolated polynucleotide comprising a nucleotide sequence which has at least 70% identity to SEQ ID NO:6, said identity being calculated over the entire length of SEQ ID NO:6.
An expression vector comprising a polynucleotide, wherein said expression vector is capable of producing an IAP polypeptide, comprising the amino acid sequence of SEQ ID NO:7 when said expression system is present in a compatible host cell.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:7.
An isolated polynucleotide, comprising a nucleotide sequence that has at least 70% identity to a nucleotide sequence encoding the IAP polypeptide of SEQ ID NO:9, said identity being over the entire region encoding for SEQ ID NO:9.
An isolated polynucleotide, comprising a nucleotide sequence that has at least 70% identity to SEQ ID NO:8, said identity being calculated over the entire length of SEQ ID NO:8.
An expression vector comprising a polynucleotide, wherein said expression vector is capable of producing an IAP polypeptide comprising the amino acid sequence of SEQ ID NO:9 when said expression system is present in a compatible host cell.
An isolated polypeptide, comprising the amino acid sequence of SEQ ID NO:9.
An isolated polynucleotide encoding a soluble protein that binds IAP.
A method of detecting organ failure caused by ischemia, reperfusion or hypoxia in a mammal, the method comprising the steps of:
A method of detecting organ failure caused by ischemia, reperfusion or hypoxia in a mammal, the method comprising the steps of:
A method of detecting a condition that is related to an increased inflammatory response in a mammal, the method comprising the steps of:
Dependencies are inferred from the claim text.