Human RNase H1 mutants
AssigneeIsis Pharmaceuticals, Inc.
InventorsHongjiang Wu, Walter F. Lima, Stanley T. Crooke
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Abstract
A human RNase H polypeptide and methods of use are provided.
Claims42
A human RNase H1 polypeptide which comprises one or more mutations compared to wild type human RNase H1, wherein the mutant version retains detectable cleavage activity for the RNA strand of an RNA/DNA duplex.
A human RNase H1 polypeptide which consists solely of the basic substrate binding domain (region III) and which retains detectable cleavage activity for the RNA strand of an RNA/DNA duplex.
A human RNase H1 polypeptide which comprises one or more mutations compared to wild type human RNase H1, wherein the mutant version retains detectable cleavage activity for the RNA strand of an RNA/DNA duplex and wherein the cleavage pattern obtained is substantially identical to that of a wild type human RNase H1.
A composition comprising a human RNase H1 polypeptide which comprises one or more mutations compared to wild type human RNase H1, wherein the mutant version retains detectable cleavage activity for the RNA strand of an RNA/DNA duplex and wherein the cleavage pattern obtained is broader than that of a wild type human RNase H1.
A human RNase H1 polypeptide which comprises one or more mutations compared to wild type human RNase H1, wherein the mutant version retains no detectable cleavage activity for the RNA strand of an RNA/DNA duplex.
Dependencies are inferred from the claim text.