Recombinant n-smases and nucleic acids encoding same
OwnerSubroto Chatterjeeinventor
Life of this patent
Estimating the term…
Abstract
Isolated nucleic acids are provided that encode human neutral sphingomyelinase (N-SMase) and N-SMase fragments and derivatives capable of hybridizing to such N-SMase-encoding nucleic acids. The invention also includes isolated recombinant human neutral sphingomyelinase (N-SMase) and N-SMase fragments and derivatives are also provided. Novel assays are also provided to identify compounds useful in the diagnosis or treatment of human neutral sphingomyelinase related disorders.
Claims30
An isolated nucleic acid encoding human neutral sphingomyelinase.
A nucleic acid that hybridizes to the sequence of SEQ ID NO:1 under normal stringency conditions.
A method of identifying a compound useful in the diagnosis or treatment of a human neutral sphingomyelinase related disorder, comprising contacting a candidate pharmacological agent with human neutral sphingomyelinase or fragment or derivative thereof and analyzing the mixture of the candidate agent and human neutral sphingomyelinase or fragment or derivative thereof.
An isolated human neutral sphingomyelinase having an apparent molecular weight of about 44 kDa as determined by polyacrylamide gel electrophoresis using sodium laurylsarocine.
An isolated polypeptide having at least about 70 percent sequence identity to SEQ ID NO:2.
A method of maintaining samples of sperm or seminal fluid comprising providing a mixture comprising sperm or seminal fluid and an effective amount of an a fragment or derivative of human neutral sphingomyelinase.
A method to reduce TNF-α induced apoptosis of mammalian cells comprising administering to a mammal an effective amount of antibody against N-SMase or fragment or derivative thereof.
Dependencies are inferred from the claim text.