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US 2002/0160482 A1Published application
Methods for protein purification
OwnerLars Abrahmsen, Joakim Nilssoninventors
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Abstract
The present invention relates to a recombinant construct comprising a nucleotide sequence encoding a fusion protein comprising a soluble form of human SSAO (Semicarbazide-Sensitive Amine Oxidase), a secretable fusion partner, a signal peptide; and a protease cleavage site. The said construct is useful in methods for purification of a soluble form of human SSAO.
Claims26
A nucleic acid comprising a nucleotide sequence encoding a secreted fusion protein comprising:
- (i) a signal peptide that directs secretion of the fusion protein from a host cell;
- (ii) a soluble form of human semicarbazide-sensitive amine oxidase (SSAO);
- (iii) a fusion partner that enables dimerization of the soluble form of human SSAO;
- and
- (iv) a protease cleavage site located between the soluble form of human SSAO and the fusion partner.
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Dependencies are inferred from the claim text.