Erythrose reductase, its cDNA and cell which the cDNA express
AssigneeDirector of National Food Research Institute
InventorsTetuya Ookura, Takafumi Kasumi, Eiji Asaba
Life of this patent
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Abstract
Protein having an erythrose reductase activity; a DNA encoding the protein; a cell to which a DNA has been transferred in a manner such that the DNA is capable of expressing an erythrose reductases type III, II or I the DNA encodes; and a method for producing erythritol, comprising acting the erythrose reductases type III, II or I or a cell to which erythrose reductases type III, II or I has been transferred in a manner capable of expressing the erythrose reductases type III, II or I on D-erythrose and harvesting the produced erythritol.
Claims26
A protein shown in (A) or (B) below:
- (A) a protein having an amino acid sequence of SEQ. ID No. 1 in the Sequence Listing;
- (B) a protein having an amino acid sequence of SEQ. ID No. 1 in the Sequence Listing, wherein the amino acid sequence includes substitution, deletion, insertion, addition or inversion of one or several amino acids and wherein the protein has an erythrose reductase activity.
A DNA encoding a protein shown in (A) or (B) below:
- (A) a protein having an amino acid sequence of SEQ. ID No. 1 in the Sequence Listing;
- (B) a protein having an amino acid sequence of SEQ. ID No. 1 in the Sequence Listing, wherein the amino acid sequence includes substitution, deletion, insertion, addition or inversion of one or several amino acids and wherein the protein has an erythrose reductase activity.
A protein shown in (C) or (D) below:
- (C) a protein having an amino acid sequence of SEQ. ID No. 2 in the Sequence Listing;
- (D) a protein having an amino acid sequence of SEQ. ID No. 2 in the Sequence Listing, wherein the amino acid sequence includes substitution, deletion, insertion, addition or inversion of one or several amino acids and wherein the protein has an erythrose reductase activity.
A DNA encoding a protein shown in (C) or (D) below:
- (C) a protein having an amino acid sequence of SEQ. ID No. 2 in the Sequence Listing;
- (D) a protein having an amino acid sequence of SEQ. ID No. 2 in the Sequence Listing, wherein the amino acid sequence includes substitution, deletion, insertion, addition or inversion of one or several amino acids and wherein the protein has an erythrose reductase activity.
A protein shown in (E) or (F) below:
- (E) a protein having an amino acid sequence of SEQ. ID No. 3 in the Sequence Listing;
- (F) a protein having an amino acid sequence of SEQ. ID No. 3 in the Sequence Listing, wherein the amino acid sequence includes substitution, deletion, insertion, addition or inversion of one or several amino acids and wherein the protein has an erythrose reductase activity.
A DNA encoding a protein shown in (E) or (F) below:
- (E) a protein having an amino acid sequence of SEQ. ID No. 3 in the Sequence Listing;
- (F) a protein having an amino acid sequence of SEQ. ID No. 3 in the Sequence Listing, wherein the amino acid sequence includes substitution, deletion, insertion, addition or inversion of one or several amino acids and wherein the protein has an erythrose reductase activity.
Dependencies are inferred from the claim text.