Phagemid for antibody screening
AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
InventorsFrank Breitling, Melvyn Little, Stefan Dubel, Michael Braunagel, Iris Klewinghaus
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Abstract
A phagemid has been constructed that expresses an antibody fused to coliphage pIII protein. The phagemid is suitable for selecting specific antibodies from large gene libraries with small quantities of antigen. The antibody-pIII gene can be strongly repressed, so that it allows antibody libraries to be amplified without the danger of deletion mutants predominating. After induction, large quantities of the fusion protein may be expressed.
Claims12
A phagemid comprising DNA encoding a polypeptide-coliphage pIII fusion protein, wherein said fusion protein comprises a polypeptide and a coliphage pIII polypeptide and wherein said coliphage pIII polypeptide comprises contiguous amino and carboxy domains of a coliphage pIII protein.
A method of presenting a polypeptide at the surface of a phagemid viral particle, comprising producing said phagemid viral particle, wherein said phagemid viral particle comprises a DNA sequence encoding said polypeptide fused to a DNA sequence encoding a coliphage pIII polypeptide comprising contiguous amino and carboxy domains of a coliphage pIII protein.
Dependencies are inferred from the claim text.