Process for production of n-glucosamine
OwnerAlan Berry, Richard P. Burlingame +1inventors
InventorsALAN BERRY, RICHARD P. BURLINGAME, JAMES R. MILLIS
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Abstract
The present invention relates to a method for producing N-glucosamine by fermentation of a genetically modified microorganism.
Claims39
A method to produce N-glucosamine by fermentation, comprising:
- (a) culturing in a fermentation medium comprising assimilable sources of carbon, nitrogen and phosphate, a microorganism having a genetic modification in an amino sugar metabolic pathway, said amino sugar metabolic pathway selected from the group consisting of a pathway for converting N-glucosamine-6-phosphate into another compound, a pathway for synthesizing N-glucosamine-6-phosphate, a pathway for transport of N-glucosamine or N-glucosamine-6-phosphate out of said microorganism, a pathway for transport of N-glucosamine into said microorganism, and a pathway which competes for substrates involved in the production of N-glucosamine-6-phosphate;
- wherein said step of culturing produces a product selected from the group consisting of N-glucosamine-6-phosphate and N-glucosamine from said microorganism;
- and
- (b) recovering said product.
A method to produce N-glucosamine by fermentation, comprising:
- (a) culturing in a fermentation medium comprising assimilable sources of carbon, nitrogen and phosphate, an Escherichia coli transformed with a recombinant nucleic acid molecule encoding N-glucosamine-6-phosphate synthase, wherein said recombinant nucleic acid molecule increases expression of said N-glucosamine-6-phosphate synthase by said Escherichia coli, and wherein said recombinant nucleic acid molecule is operatively linked to a transcription control sequence;
- wherein said step of culturing produces a product selected from the group consisting of N-glucosamine-6-phosphate and N-glucosamine from said Escherichia coli;
- and
- (b) recovering said product.
A microorganism for producing N-glucosamine by a biosynthetic process, said microorganism being transformed with a recombinant nucleic acid molecule encoding N-glucosamine-6-phosphate synthase, said recombinant nucleic acid molecule being operatively linked to a transcription control sequence and comprising a genetic modification which reduces N-glucosamine-6-phosphate product inhibition of said N-glucosamine-6-phosphate synthase;
- wherein expression of said recombinant nucleic acid molecule increases expression of said N-glucosamine-6-phosphate synthase by said microorganism.
A microorganism for producing N-glucosamine by a biosynthetic process, said microorganism comprising:
- (a) a recombinant nucleic acid molecule encoding N-glucosamine-6-phosphate synthase, said recombinant nucleic acid molecule being operatively linked to a transcription control sequence, wherein expression of said recombinant nucleic acid molecule increases expression of said N-glucosamine-6-phosphate synthase by said microorganism;
- and,
- (b) at least one genetic modification in a gene encoding a protein selected from the group consisting of N-acetylglucosamine-6-phosphate deacetylase, N-glucosamine-6-phosphate deaminase, N-acetyl-glucosamine-specific enzyme IINag, phosphoglucosamine mutase, N-glucosamine-1-phosphate acetyltransferase-N-acetylglucosamine-1-phosphate uridyltransferase, phosphofructokinase, Enzyme IIGlc of the PEP:glucose PTS, EIIM, P/IIIMan of the PEP:mannose PTS, and alkaline phosphatase, wherein said genetic modification decreases enzymatic activity of said protein.
Dependencies are inferred from the claim text.