Ups (ugc)
OwnerJianzhong Huang, Xinhe Jiang +2inventors
InventorsJianzhong Huang, Xinhe Jiang, Damien McDevitt, Christopher M. Traini
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Abstract
The invention provides ups (ugc) polypeptides and polynucleotides encoding ups (ugc) polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ups (ugc) polypeptides to screen for antibacterial compounds.
Claims16
An isolated polypeptide selected from the group consisting of:
- (i) an isolated polypeptide comprising an amino acid having at least:
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95%identity to the amino acid sequence of SEQ ID NO:2 over the entire length of SEQ ID NO:2;
- (ii) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2,
- (iii) an isolated polypeptide which is the amino acid sequence of SEQ ID NO:2, and
- (iv) a polypeptide which is encoded by a recombinant polynucleotide comprising the polynucleotide sequence of SEQ ID NO:1.
An isolated polynucleotide selected from the group consisting of:
- (i) an isolated polynucleotide comprising a polynucleotide sequence ending a polypeptide that has at least
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity;
- to the amino acid sequence of SEQ ID NO:2, over the entire length of SEQ ID NO:2;
- (ii) an isolated polynucleotide comprising a polynucleotide sequence that has at least:
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity;
- over its entire length to a polynucleotide sequence encoding the polypeptide of SEQ ID NO:2;
- (iii) an isolated polynucleotide comprising a nucleotide sequence which has at least:
- (a) 70% identity;
- (b) 80% identity;
- (c) 90% identity;
- or
- (d) 95% identity;
- to that of SEQ ID NO:1 over the entire length of SEQ ID NO:1;
- (iv) an isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO:2;
- (v) an isolated polynucleotide which is the polynucleotide of SEQ ID NO:
- 1;
- (vi) an isolated polynucleotide obtainable by screening an appropriate library under stringent hybridization conditions with a probe having the sequence of SEQ ID NO:
- 1 or a fragment thereof;
- (vii) an isolated polynucleotide encoding a mature polypeptide expressed by the ups (ugc) gene contained in the Pseudomonas aeruginosa;
- and
- (viii) a polynucleotide sequence complementary to said isolated polynucleotide of (i), (ii), (iii), (iv), (v), (vi) or (vii).
A computer readable medium having stored thereon a member selected from the group consisting of:
- a polynucleotide comprising the sequence of SEQ ID NO:1;
- a polypeptide comprising the sequence of SEQ ID NO:2;
- a set of polynucleotide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO:1;
- a set of polypeptide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO:2;
- a data set representing a polynucleotide sequence comprising the sequence of SEQ ID NO:1;
- a data set representing a polynucleotide sequence encoding a polypeptide sequence comprising the sequence of SEQ ID NO:2;
- a polynucleotide comprising the sequence of SEQ ID NO:1;
- a polypeptide comprising the sequence of SEQ ID NO:2;
- a set of polynucleotide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO:
- 1;
- a set of polypeptide sequences wherein at least one of said sequences comprises the sequence of SEQ ID NO:2;
- a data set representing a polynucleotide sequence comprising the sequence of SEQ ID NO:
- 1;
- a data set representing a polynucleotide sequence encoding a polypeptide sequence comprising the sequence of SEQ ID NO:2.
A computer based method for performing homology identification, said method comprising the steps of providing a polynucleotide sequence comprising the sequence of SEQ ID NO:
- 1 in a computer readable medium;
- and comparing said polynucleotide sequence to at least one polynucleotide or polypeptide sequence to identify homology.
A further embodiment of the invention provides a computer based method for polynucleotide assembly, said method comprising the steps of:
- providing a first polynucleotide sequence comprising the sequence of SEQ ID NO:1 in a computer readable medium;
- and screening for at least one overlapping region between said first polynucleotide sequence and a second polynucleotide sequence.
A polynucleotide consisting of a polynucleotide of the formula:
- X—(R1)m—(R2)—(R3)n—Y
- wherein, at the 5′ end of the molecule, X is hydrogen, a metal or a modified nucleotide residue, or together with Y defines a covalent bond, and at the 3′ end of the molecule, Y is hydrogen, a metal, or a modified nucleotide residue, or together with X defines the covalent bond, each occurrence of R1 and R3 is independently any nucleic acid residue or modified nucleic acid residue, m is an integer between 1 and 3000 or zero, n is an integer between 1 and 3000 or zero, and R2 is a nucleic acid sequence or modified nucleic acid sequence set forth in SEQ ID NO:1.
Dependencies are inferred from the claim text.