Method for binding basophils and mast cells
AssigneeEberhard-Karls-Universität Tübingen Universitaäsklinikum
InventorsHans-Jorg Buhring, Johannes Andreas Van Agthoven, David Jarossay
Life of this patent
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Abstract
The present invention concerns an antibody for the detection and/or quantification and/or isolation of basophils and/or mast cells and/or of the precursor cells of basophils and/or mast cells and/or of a surface structure of said cells, binding of the antibody occurring to that surface structure of the cells to which can bind the antibody with the designation 97A6, produced and released by hybridoma cells that were deposited in accordance with the Budapest Treaty on Feb. 12, 1997 under number DSM ACC 2297 at Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ).
Claims21
A method for binding cells, comprising contacting said cells with an antibody that binds to a surface structure on said cells, wherein said surface structure is also capable of binding antibody 97A6, which is produced by the hybridoma on deposit as No. DSM ACC 2297, and wherein said cells are selected from the group consisting of basophils, mast cells, precursor cells of basophils, and precursor cells of mast cells.
A method for analyzing a patient sample comprising cells with a surface structure, comprising contacting said patient sample with an antibody capable of binding to the surface structure of said cells, wherein said surface structure is also capable of binding to antibody 97A6, which is produced by the hybridoma on deposit as No. DSM ACC 2297, and wherein the patient sample is selected from the group consisting of tissue biopsies, bone marrow biopsies and blood samples.
A method for the diagnostic classification of a tumor, comprising providing a sample of said tumor, which comprises cells having a surface structure, and contacting said surface structure with an antibody capable of binding said surface structure, wherein said surface structure is also capable of binding to antibody 97A6, which is produced by the hybridoma on deposit as No. DSM ACC 2297.
A method for investigating allergies, comprising pre-incubating a blood sample comprising cells with an agent that is suspected of triggering an allergic reaction;
- incubating said pre-incubated blood sample with an antibody capable of binding to a surface structure of said cells, wherein said surface structure is also capable of binding to antibody 97A6, which is produced by the hybridoma on deposit as No. DSM ACC 2297;
- and quantifying antibodies bound to cells in said blood sample.
A method of providing hematopoietic precursor cells that can differentiate into mast cells or basophils, comprising:
- providing bone marrow cells having a surface structure from an organism;
- incubating said bone marrow cells with an antibody capable of binding to said surface structure, wherein said surface structure is also capable of binding to antibody 97A6, which is produced by the hybridoma on deposit as No. DSM ACC 2297;
- and isolating antibody-bound cells from the incubated bone marrow cells.
A substantially pure population of cells, wherein said cells are capable of being bound by a reagent that binds specifically to a surface structure on said cells, wherein said surface structure is also capable of binding to antibody 97A6, which is produced by the hybridoma on deposit as No. DSM ACC 2297;
- and wherein said cells are selected from the group consisting of basophils, mast cells, precursor cells of basophils, and precursor cells of mast cells.
A reagent for binding cells, comprising an antibody capable of binding to a surface structure of said cells, wherein said surface structure is also capable of binding to the antibody 97A6, and wherein said cells are selected from the group consisting of basophils, mast cells, precursor cells of basophils, and precursor cells of mast cells.
Dependencies are inferred from the claim text.