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AssigneeSenomyx, Inc.
InventorsJon Elliot Adler, Xiaodong Li, Lena Staszewski, Hong Xu, Fernando Echeverri
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Newly identified mammalian taste-cell-specific G protein-coupled receptors which function as hetero-oligomeric complexes in the sweet taste transduction pathway, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in sweet taste signaling as hetero-oligomeric complexes, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for identifying putative taste modulating compounds using such hetero-oligomeric complexes also described, as is a novel surface expression facilitating peptide useful for targeting integral plasma membrane proteins to the surface of a cell.
A method of screening for compounds that modulate sweet taste signaling comprising:
A method of screening for compounds that modulate taste signaling transduction comprising:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling comprising the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling comprising the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling comprising the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling comprising the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the G protein-coupled receptor polypeptide active in taste signaling comprising the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the G protein-coupled receptor polypeptide active in taste signaling comprising the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the G protein-coupled receptor polypeptide active in taste signaling comprising the amino acid sequence of SEQ ID NO:
An isolated polypeptide selected from the group consisting of:
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
A method for enhance surface expression of integral plasma membrane proteins comprising fusing a PDZ-interacting peptide to an integral plasma membrane protein to form a heterologous protein, and expressing the heterologous protein in a cell.
An expression vector comprising an isolated nucleic acid molecule encoding for a G-protein coupled receptor polypeptide fused to a surface expression facilitating sequence having an SEQ ID NO 10, wherein said vector is selected from the group consisting of mammalian vectors, bacterial plasmids, bacterial phagemids, mammalian viruses and retroviruses, bacteriophage vectors and linear or circular DNA molecules capable of integrating into a host cell genome.
An isolated nucleic acid molecule selected from the group consisting of:
An isolated nucleic acid molecule consisting essentially of a nucleic acid sequence coding for a mammalian G protein-coupled receptor having an amino acid sequence selected from the group consisting of SEQ ID NOs 2, 4, and 6.
An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs 1, 3, 5, and 7.
An isolated variant molecule comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs 2, 4, and 6, containing at least one conservative substitution in a coding region.
Dependencies are inferred from the claim text.