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OwnerYasuyuki Nozaki, Shingo Ueno +3inventors
InventorsYasuyuki Nozaki, Shingo Ueno, Ryo Nakashige, Toshiko Matsumoto, Takuro Tamura
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A method of conducting accurate identification of biological species with a biochip, and a method of effectuating identification of biological species at a level higher than species are provided. Selection of specific probes for multiple biological species is also facilitated. A plurality of partial sequences A, A′; B, B′; and so on, which are specific to respective targets, are selected as probes in a manner that the partial sequences do not overlap one another. In addition, DNA regions I, J, K and L, which are common to some targets, respectively, are also selected as probes. Alternatively, if there is a common base sequence at leaves below a certain node based on a dendrogram of targets, such a base sequence is designed as a probe unique to the node. By using a set of probes including the probes unique to the targets and the probes unique to leaves, identification of biological species can be performed accurately, and identification of biological species at a level higher than species is also effectuated. In a case where the base sequences corresponding to leaves are identical or similar to each other, such base sequences can be used as probes if sequences corresponding to nodes are different. Therefore, selection of specific probes among multiple biological species can be facilitated.
A biochip having a substrate with a plurality of probes spotted thereon, wherein a plurality of types of probes are spotted with respect to one target so that the probes hybridize respectively with a plurality of partial sequences specific to the target, the partial sequences not overlapping each other on a base sequence of the target.
A biochip having a substrate with a plurality of probes spotted thereon, wherein a probe is spotted so that the probe hybridizes specifically with a partial sequence existing in common to base sequences of a plurality of different targets.
A biochip having a substrate with a plurality of probes spotted thereon,
A biochip having a substrate with a plurality of probes spotted thereon for discriminating a plurality of types of target biopolymers, wherein a probe hybridizing in common only with biopolymers below a node on a molecular dendrogram of a group of biopolymers including the plurality of types of target biopolymers is spotted as a probe corresponding to the node of the molecular dendrogram.
A biochip having a substrate with a plurality of probes spotted thereon for discriminating a plurality of types of target biopolymers,
A biochip having a substrate with a plurality of probes spotted thereon for discriminating a plurality of types of target biopolymers,
A probe designing method, wherein a plurality of probes are designed as probes to be spotted on a substrate of a biochip so that the probes hybridizing respectively with a plurality of partial sequences specific to a target, the partial sequences not overlapping each other on a base sequence of the target.
A probe designing method, wherein a probe is designed as a probe to be spotted on a substrate of a biochip so that the probe hybridizes specifically with a partial sequence existing in common to base sequences of a group of targets composed of a plurality of different targets.
A probe designing method,
A probe designing method for discriminating a plurality of types of target biopolymers contained in a sample, wherein a probe hybridizing in common only with biopolymers below a node on a molecular dendrogram of a group of biopolymers including the plurality of types of target biopolymers is designed as a probe corresponding to the node of the molecular dendrogram.
A probe designing method for discriminating a plurality of types of target biopolymers contained in a sample,
A probe designing method for discriminating a plurality of types of target biopolymers contained in a sample,
A target detecting method for detecting existence of a target biopolymer based on hybridization reactions with probes,
Dependencies are inferred from the claim text.