Oncoprotein protein kinase
AssigneeThe Regents of the University of California
InventorsMichael Karin, Masahiko Hibi, Anning Lin
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Abstract
An isolated polypeptide (JNK characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of detection of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.
Claims4
A method for identifying a compound which affects the activity of JNK Protein Kinase characterized by its ability to phosphorylate the c-Jun N-terminal activation domain comprising:
- a) incubating the compound and the kinase or a polynucleotide encoding the kinase, wherein the incubation is carried out under conditions sufficient to allow the components to interact;
- and
- b) measuring the effect of the compound on the kinase or polynucleotide encoding the kinase.
A method for identifying a compound which affects the activity of a JNK protein kinase characterized by its ability to phosphorylate the c-Jun N-terminal activation domain comprising:
- a) administering the compound to T cells expressing the kinase which are cultured under conditions sufficient to allow the components to interact;
- and
- b) measuring the effect of the compound on the phosphorylated state of c-Jun.
Dependencies are inferred from the claim text.