Stress-regulated genes of plants, transgenic plants containing same, and methods of use

A method of identifying a stress condition to which a plant cell has been exposed, the method comprising:

• a) contacting nucleic acid molecules representative of expressed polynucleotides in the plant cell with an array of probes representative of the plant cell genome;
• and
• b) detecting a profile of expressed polynucleotides in the plant cell characteristic of a stress response, thereby identifying the stress condition to which the plant cell was exposed.

A method of producing a transgenic plant comprising plant cells that exhibit altered responsiveness to at least one stress condition, the method comprising introducing a polynucleotide portion of a plant stress-regulated gene into a plant cell genome, wherein the polynucleotide portion of the stress-regulated gene comprises a nucleotide sequence as set forth in any of 1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1262, 1264-1386, 1387-1390, 1392-1404, 1406-1444, 1446-1483, 1485-1588, 1590-1608, 1610-1633, 1634-1725, 1727-1865, 1867-1917, 1919-1927, 1929-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3313-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, and 4604-5379, whereby the polynucleotide portion of the plant stress-regulated gene modulates a response of the plant cells to at least one stress condition, thereby producing a transgenic plant comprising plant cells that exhibit altered responsiveness to the stress condition.

A transgenic plant, which contains a transgene comprising a polynucleotide portion of plant stress-regulated gene, wherein the gene comprises a nucleotide sequence as set forth in any of SEQ ID NOS:1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1262, 1264-1386, 1387-1390, 1392-1404, 1406-1444, 1446-1483, 1485-1588, 1590-1608, 1610-1633, 1634-1725, 1727-1865, 1867-1917, 1919-1927, 1929-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3313-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, and 4604-5379.

A plant stress-regulated gene regulatory element, wherein the gene comprises a nucleotide sequence as set forth in any of SEQ ID NOS:1-155, 157-228, 230-232, 234-557, 559-572, 574-605, 607-634, 636-786, 788-812, 814-1262, 1264-1386, 1387-1390, 1392-1404, 1406-1444, 1446-1483, 1485-1588, 1590-1608, 1610-1633, 1634-1725, 1727-1865, 1867-1917, 1919-1927, 1929-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3271, 3273-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3313-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, and 4604-5379.

The plant stress-regulated gene regulatory element of claim 144, comprising a nucleotide sequence as set forth in any of SEQ ID NOS:2704-2855, 2857-2928, 2930-2932, 2934-3256, 3258-3304, 3306-3323, 3325-3333, 3335-3485, 3487-3511, 3513-3956, 3958-4078, 4080-4097, 4099-4136, 4138-4175, 4177-4279, 4281-4299, 4301-4324, 4326-4414, 4416-4552, 4554-4602, 4604-4612, and 4614-5379.

A method for identifying a polynucleotide involved in a stress response of a plant, the method comprising:

• a) contacting nucleic acid molecules representative of expressed polynucleotides in plant cells of a plant exposed to a stress condition or combination of stress conditions with an array of probes representative of the plant cell genome;
• and
• b) detecting a nucleic acid molecule that exhibits at least a two-fold change in the level of expression as compared to the level of the nucleic acid molecule in a corresponding plant cell of a plant that was not exposed to the stress condition, thereby identifying a polynucleotide involved in a stress response of the plant.

A computer readable medium having stored thereon computer executable instructions for performing a method comprising:

• a) receiving data on expression in a cell of a plant of a nucleic acid molecule having at least 70% sequence identity to a nucleotide sequence comprising any of SEQ ID NO. 1-5379;
• and
• b) comparing the data on expression of the nucleic acid molecule with data on expression of the nucleic acid in a cell of a plant that has not been exposed to an abiotic stress, of a plant that has been exposed to an abiotic stress condition or combination of abiotic stress conditions, or of a combination of such plants.

A computer-readable medium having stored thereon a data structure comprising:

• sequence data for at least one nucleic acid molecule having at least 70% nucleic acid sequence identity to a polynucleotide having a nucleotide sequence as set forth in any of SEQ ID NO. 1-5379 or a nucleotide sequence complementary thereto;
• and
• a module receiving the nucleic acid molecule sequence data, which compares the nucleic acid molecule sequence data to a least one other nucleic acid sequence.