Biomaterial derived from vertebrate liver tissue
AssigneePurdue Research Foundation
InventorStephen F. Badylak
Life of this patent
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Abstract
A tissue graft composition comprising liver basement membrane is described. The graft composition can be implanted to replace or induce the repair of damaged or diseased tissues.
Claims16
A composition comprising liver basement membrane of a warm-blooded vertebrate, wherein the liver basement membrane is substantially free of cells of said warm-blooded vertebrate.
A composition prepared from liver tissue of a warm-blooded vertebrate, wherein said composition is useful as a non-immunogenic tissue graft capable of inducing endogenous tissue growth when implanted in warm-blooded vertebrates, said composition comprising an extracellular matrix consisting essentially of liver basement membrane devoid of endogenous cells associated with said liver tissue.
The use of liver basement membrane to manufacture a non-immunogenic tissue graft composition for use in the repair of damaged or diseased tissues.
A method for inducing the formation of endogendus tissue at a site in need of endogenous tissue growth in a warm blooded vertebrate, said method comprising implanting a graft composition comprising an extracellular matrix consisting essentially of basement membrane of liver tissue of a warm-blooded vertebrate in an amount effective to induce endogenous tissue growth at the site the graft composition is administered.
A method for preparing a tissue graft composition from warm-blooded vertebrate liver tissue having both cellular and extracellular components, said method comprising the steps of
- treating the liver tissue with a cell dissociation solution for a period of time sufficient to release the cellular components of the liver tissue from the extracellular components without substantial disruption of the extracellular components, and
- separating the cellular components from said extracellular components.
A liver tissue derived composition for supporting the growth of a cell population, said composition comprising isolated liver basement membrane devoid of source liver tissue endogenous cells;
- and
- added nutrients to support the growth of said cell population in vitro.
A liver tissue derived composition for supporting the growth of a cell population, said composition comprising
- culture-ware coated with a matrix comprising liver basement membrane devoid of source liver tissue endogenous cells.
Dependencies are inferred from the claim text.