Methods of treating lymphoma and leukemia
AssigneeGenitope Corporation
InventorDan W. Denney JR.
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Abstract
The present invention provides multivalent vaccines for the treatment of B-cell malignancies (e.g., lymphomas and leukemias). The present invention also provides methods for the production of custom vaccines, including multivalent vaccines for the treatment of simmune cell tumors malignancies as well as methods of treating immune cell tumors using custom vaccines.
Claims24
A multivalent vaccine comprising at least two recombinant variable regions of immunoglobulin molecules derived from B-cell lymphoma cells, wherein said cells express at least two different immunoglobulin molecules, said immunoglobulin molecules differing by at least one idiotope.
A method of producing a vaccine for treatment of B-cell lymphoma comprising:
- a) providing:
- i) malignant cells isolated from a patient having a B-cell lymphoma;
- ii) an amplification vector comprising a recombinant oligonucleotide having a sequence encoding a first inhibitable enzyme operably linked to a heterologous promoter;
- iii) a eukaryotic parent cell line;
- b) isolating from said malignant cells nucleotide sequences encoding at least one VH region and at least one VL region, said VH and VL regions derived from immunoglobulin molecules expressed by said malignant cells;
- c) inserting said nucleotide sequences encoding said VH and VL regions into at least one expression vector;
- d) introducing said at least one expression vector and said amplification vector into said parent cell to generate one or more transformed cells;
- e) growing said transformed cell in a first aqueous solution containing an inhibitor capable of inhibiting said inhibitable enzyme wherein the concentration of said inhibitor present in said first aqueous solution is sufficient to prevent growth of said parent cell line;
- and
- f) identifying a transformed cell capable of growth in said first aqueous solution, wherein said transformed cell capable of growth expresses said VH and VL regions.
A method of treating B-cell lymphoma, comprising:
- a) providing:
- i) a subject having a B-cell lymphoma;
- ii) a multivalent vaccine comprising at least two recombinant variable regions of immunoglobulin molecules derived from said subjects's B-cell lymphoma cells, wherein said cells express at least two different immunoglobulin molecules, said immunoglobulin molecules differing by at least one idiotope;
- b) administering said multivalent vaccine to said subject.
Dependencies are inferred from the claim text.