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AssigneeInhibitex, Inc.
InventorsJoseph M. Patti, Timothy J. Foster, Magnus Hook
Estimating the term…
A method and composition for the passive immunization of patients infected with or susceptible to infection from Staphylococcus bacteria such as S. aureus and S. epidermidis infection is provided that includes the selection or preparation of a donor plasma pool with high antibody titers to carefully selected Staphylococcus adhesins or MSCRAMMs, or fragments or components thereof, or sequences with substantial homology thereto. The donor plasma pool can be prepared by combining individual blood or blood component samples which have higher than normal titers of antibodies to one or more of the selected adhesins or other proteins that bind to extracellular matrix proteins, or by administering carefully selected proteins or peptides to a host to induce the expression of desired antibodies, and subsequently recovering the enhanced high titer serum or plasma pool from the treated host. In either case, the donor plasma pool is preferably purified and concentrated prior to intravenous introduction into the patient, and the present invention is advantageous in that a patient can be immunized against a wide variety of potentially dangerous staphylococcal infections. Kits for identifying potential donor with high titers of the selected adhesins are also provided. The present invention thus provides methods and compositions which can be highly effective against infections associated with Staphylococcus bacteria.
A method of obtaining an immunoglobulin composition having a higher than normal antibody titer to a clumping factor A (ClfA) adhesin comprising obtaining blood or plasma samples from donors, identifying those blood or plasma samples from high-titer donors having the presence of an antibody titer to ClfA in an amount which is higher than that found in pooled intravenous immunoglobulin obtained from unselected donors, recovering blood or plasma from the identified high-titer donors, and treating the donor blood or plasma to obtain immunoglobulin in a purified state that has an antibody titer to ClfA in an amount which is higher than that found in intravenous immunoglobulin obtained from unselected donors.
A method of obtaining an immunoglobulin composition having a higher than normal antibody titer to a clumping factor A (ClfA) adhesin comprising administering ClfA to a host donor in an amount sufficient so as to induce an antibody titer to ClfA in an amount which is higher than that found in pooled intravenous immunoglobulin obtained from unselected donors, recovering blood or plasma from the host donor, and treating the donor blood or plasma to obtain immunoglobulin in a purified state that has an antibody titer to ClfA which is higher than that found in pooled intravenous immunoglobulin obtained from unselected donors.
A method of obtaining an immunoglobulin composition having a higher than normal antibody titer to a staphylococcal Sdr protein comprising obtaining blood or plasma samples from donors, identifying those blood or plasma samples from high-titer donors having the presence of an antibody titer to a staphylococcal Sdr protein in an amount which is higher than that found in pooled intravenous immunoglobulin obtained from unselected donors, recovering blood or plasma from the identified high-titer donors, and treating the donor blood or plasma to obtain immunoglobulin in a purified state that has an antibody titer to a staphylococcal Sdr protein in an amount which is higher than that found in intravenous immunoglobulin obtained from unselected donors.
A method of obtaining an immunoglobulin composition having a higher than normal antibody titer to a staphylococcal Sdr protein comprising administering a staphylococcal Sdr protein to a host donor in an amount sufficient so as to induce an antibody titer to a staphylococcal Sdr protein in an amount which is higher than that found in pooled intravenous immunoglobulin obtained from unselected donors, recovering blood or plasma from the host donor, and treating the donor blood or plasma to obtain immunoglobulin in a purified state that has an antibody titer to a staphylococcal Sdr protein which is higher than that found in pooled intravenous immunoglobulin obtained from unselected donors.
Dependencies are inferred from the claim text.