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US 2002/0156240 A1Published application
Alpha-agarase and process for producing the same
AssigneeTakara Shuzo Co., Ltd.
InventorsJun Tomono, Yoshiko Nomura, Hiroaki Sagawa, Takeshi Sakai, Ikunoshin Kato
Life of this patent
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Abstract
A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.
Claims23
An α-agarase having the following physical and chemical properties:
- (1) action:
- hydrolyzing an α-1,3 bond between 3,6-anhydro-L-galactose and D-galactose;
- (2) substrate specificity:
- acting on agarose, agarohexaose and agarooligosaccharides longer than agarohexaose but not on agarotetraose;
- (3) optimal temperature:
- exhibiting its enzymatic activity at a temperature of 55° C. or below;
- and
- (4) heat stability:
- retaining 20% or more of its activity after treatment at 48° C. for 30 seconds.
6 more elements
A polypeptide having an α-agarase activity, selected from the group consisting of:
- (1) a polypeptide containing an amino acid sequence consisting of 591 residues from amino acid number 335 to amino acid number 925 in the amino acid sequence of SEQ ID NO:
- 14;
- (2) a polypeptide containing an amino acid sequence in which one or more amino acids are substituted, deleted, added and/or inserted in an amino acid sequence consisting of 591 residues from amino acid number 335 to amino acid number 925 in the amino acid sequence of SEQ ID NO:
- 14;
- (3) a polypeptide containing an amino acid sequence consisting of 586 residues from amino acid number 365 to amino acid number 950 in the amino acid sequence of SEQ ID NO:
- 15;
- and
- (4) a polypeptide containing an amino acid sequence in which one or more amino acids are substituted, deleted, added and/or inserted in an amino acid sequence consisting of 586 residues from amino acid number 365 to amino acid number 950 in the amino acid sequence of SEQ ID NO:
- 15.
6 more elements
Dependencies are inferred from the claim text.