Integrated portable biological detection system

A method for performing an analysis of a biological sample having desired cells and undesired cells using an integrated portable system comprising a flow cell, a plurality of individually addressable electrodes positioned within the flow cell, and a portable housing containing the flow cell, the method comprising:

• introducing the sample into the flow cell via an input port coupled to the flow cell;
• creating a dielectric force pattern by individually biasing the electrodes positioned within the flow cell;
• subjecting the sample to the dielectric force pattern to separate the undesired cells from the desired cells in the sample;
• isolating the desired cells by maintaining an attractive bias for the desired cells and introducing a flow of wash buffer through the flow cell via the input port to eliminate the undesired cells, wherein the undesired cells are removed from the flow cell via an output port coupled to the flow cell;
• lysing the desired cells by applying an electric force within the flow cell to electronically disrupt the desired cells into a plurality of components;
• and
• analyzing the components of the desired cells by hybridizing the components with probes to form probe-target hybrids within the flow cell, and detecting the probe-target hybrids while the hybrids are within the flow cell, wherein the probes are coupled to the electrodes.

A method for performing an analysis of a biological sample having desired cells and undesired cells using an integrated portable system, the method comprising:

• providing an integrated portable system comprising an electronic system having a flow cell, a plurality of individually addressable electrodes positioned within the flow cell, a plurality of probes coupled to the electrodes, a detector configured to detect the probes, a power source coupled to the flow cell, and a portable housing containing the electronic system, the detector, and the power source;
• introducing the sample into the integrated portable system by injecting the sample into the flow cell via an input port coupled to the flow cell, wherein the input port is accessed through the portable housing;
• creating a dielectric force pattern by individually biasing the electrodes positioned within the flow cell;
• subjecting the sample to the dielectric force pattern to separate the undesired cells from the desired cells in the sample;
• isolating the desired cells by maintaining an attractive bias for the desired cells and introducing a flow of wash buffer through the flow cell via the input port to eliminate the undesired cells, wherein the undesired cells are removed from the flow cell via an output port coupled to the flow cell;
• lysing the desired cells by applying an electric force within the flow cell to electronically disrupt the desired cells into a plurality of components;
• and
• analyzing the components of the desired cells by hybridizing the components with probes to form probe-target hybrids within the flow cell, and detecting the probe-target hybrids while the hybrids are within the flow cell.