Nucleotide sequences which code for the pgsA2 gene
OwnerMadhavan Nampoothiri K., Bettina Mockel +3inventors
InventorsMadhavan Nampoothiri K., Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm
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Abstract
The invention relates to a genetically modified coryneform bacterium, the pgsA2 gene of which is amplified, and an isolated polynucleotide which codes for CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the pgsA2 gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.
Claims24
A genetically modified coryneform bacterium, wherein the pgsA2 gene, which codes for CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase, is amplified.
An isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence selected from the group consisting of
- a) a polynucleotide which is homologous to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2,
- b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is homologous to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2,
- c) a polynucleotide which is complementary to the polynucleotides of a) or b), and
- d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c).
A method for the fermentative preparation of L-amino acids, comprising:
- a) fermentating coryneform bacteria which produce L-amino acids and in which at least the pgsA2 gene or nucleotide sequences which code for it are amplified, in particular over-expressed.
Dependencies are inferred from the claim text.