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US 2002/0153321 A1Published application
Autoclavable annular chromatograph
AssigneePrior Separation Technology GmbH
InventorsAdalbert Prior, Joachim Prior, Juergen Wolfgang
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AssigneePrior Separation Technology GmbH
InventorsAdalbert Prior, Joachim Prior, Juergen Wolfgang
Estimating the term…
The invention relates to a CAC device comprising a stationary column head, a stationary eluate collecting ring, a rotating body which is located between said column head and said collecting ring and which can rotate about a vertical axis and contains a chromatographic separating medium; and a drive device. Said rotating body (1) is connected to a rotating platform (4) at its bottom end. This rotating platform (4) is slidingly, sealingly guided on the eluate collecting ring (5) and has an extension on the underside. The extension can be coupled to a drive shaft (2) of the drive device (8) with a rapidly detachable connection and the stationary column head the rotating body (1) with the rotating platform (4) and the attached eluate collecting ring (5) can be connected to form a CAC module and can be removed from the drive shaft (2) as a unit. The invention also relates to an inverse CAC method using the inventive device. According to this method, the sample material is distributed over the entire periphery of the separating medium.
Device for continuous annular chromatography (CAC) with a stationary column head, a stationary eluate collecting ring, between them a rotating body rotatable about a vertical axis and shaped as a twin cylinder forming a hollow jacket with a chromatographic separation medium contained therein, as well as a drive, the rotating body (1) being connected at its bottom end with a rotating platform (4) having vertical through channels (15) which is guided sealingly and slidingly on a stationary eluate collecting ring (5) with chambers (16), characterized in that the rotating platform (4) at its underside is provided with an extension that can be coupled to a drive shaft (2) of the drive (8) via a rapidly detachable connection, and the stationary column head, the rotating body (1) with rotating platform (4) and the adjacent eluate collecting ring (5) are combined into a CAC module and can be detached as a unit from the drive shaft (2).
Device according to one of the preceding claims, characterized in that the rotating platform (4) is supported by a peripheral axial bearing (25) borne by distance rods (24), and is slidingly guided on this bearing, preferably via a horizontal O-ring.
Device according to one of the preceding claims, characterized in that a pressure pad (7) pushing the eluate collecting ring (5) against the rotating platform (4) is arranged adjacent to the underside of the eluate collecting ring (5).
Device according to one of the preceding claims, characterized in that the rotating platform (4) rests on two concentric gliding surfaces on the eluate collecting ring (5) and the eluate collecting ring (5) between the inner gliding surface and its inner rim adjoining the drive shaft (2) has a recess beveled toward the outside which together with the rotating platform (4) forms a hollow space (20) from the lowest point of which at least one bore (21) leads from the eluate collecting ring (5) to the outside.
Device according to one of the preceding claims, characterized in that the eluate collecting ring (5) has a multitude of chambers (16) separated by radial walls, the thickness of each radial wall being smaller than the diameter of a channel (15) of the rotating platform (4).
Device according to one of the preceding claims, characterized in that eluate drain lines (18), preferably in the form of flexible hoses, are attached to the chambers (16) of the eluate collecting ring (5), these hoses first running downward, then bending up like a wave in the way of a recumbent “S”, finally bending again downward and, where appropriate, terminating in eluate collection vessels (19), where the highest part of the eluate drain line (18) preferably is above the level of the eluate collecting ring (5).
Device according to one of the preceding claims, characterized in that the inner cylinder of the twin cylinder of the rotating body (1) at its lower end is solidly connected with a bottom support (11) which in turn rests on the rotating platform (4) and is connected immovably with it by at least one connecting element, for instance a bolt, a pin, or a screw (12).
Device according to one of the preceding claims, characterized in that it has a number of extension tubes arranged in the head space above the separation medium and projecting into the annular gap of the hollow cylinder jacket of the rotating body (1), these tubes being connected with the inlet ports for the liquid supply lines (27a, 27c) and, where appropriate, able to be shifted in annular fashion along the periphery of the annular gap above the separation medium, and to be fixably arranged at desired angular positions.
Device according to one of the preceding claims, characterized in that it has means for temperature control and/or thermostatic operation, in particular a heating and/or cooling circuit arranged inside and/or outside of the rotating body (1)
Dependencies are inferred from the claim text.