Process and means for down stream processing
AssigneePharmacia LKB Biotechnology AB
InventorsMats Carlsson, Jan-Gunnar Gustafsson, Per Hedman, Per-Ake Pernemalm, Jorgen Lonngren, Annika Lindgren
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Abstract
A method for recovering a desired component from a sample solution with beads under stabilized fluidized bed conditions is provided. Also provided are support beads for use in downstream processing which comprise a polymer matrix into which glass or silica particles have been incorporated.
Claims10
A method for recovering a desired component from a sample solution comprising the steps of
- (i) feeding the sample solution comprising the desired component to be recovered into a column containing beads having affinity for the component, through a bottom port equipped with a flow distributor, under stabilized fluidized bed conditions, whereby unbound components of the sample solution are discharged through the top port of the column
- (ii) washing the column with a solution introduced through said bottom port under flow conditions, maintaining an expanded bed, whereby remaining solid components and other impurities of the sample solution are discharged through the top port of the column,
- (iii) introducing an elution solution for releasing sample components bound to the beads and collecting said components.
A method according to any one of claims 1 and 2, characterized in that the beads forming the fluidized bed are chromatographic support beads according to any one of claims 5-10.
Support beads for use in downstream processing, characterized in that they comprises a polymer matrix into which glass or silica particles have been incorporated.
Dependencies are inferred from the claim text.