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US 2002/0153294 A1Published application
Chromatography apparatus
AssigneeBiotage, Inc., a Delaware corporation
InventorsPeter J. Leavesley, Peter C. Van Davelaar, Robert D. Lockman, Ivan Hargro, Jeffrey A. Horsman, Peter C. Rahn
Life of this patent
Estimating the term…
Abstract
An apparatus for use in liquid chromatography involves a column filled with media bounded axially by first and second porous plates. The column has an inlet end with an inflow region between the inlet end and the first porous plate. The column also has an outlet end with an outflow region between the outlet end and the second porous plate. The inflow region may be substantially longer than the outflow region. The porous plates may be moveable under pressure so as to compress the media.
Claims85
An apparatus for sealing a sample-conveying conduit to a chromatography cartridge containing a media bed that forms a stop comprising:
- a first head piece and a second head piece, both of which are elongated along a longitudinal axis and are sized to fit slidably into the cartridge, said first head piece having a body, a first compression face, and a contact face adapted to contact said stop, said second head piece having a second compression face and a compression force receiving member, said body defining an elongate flow channel along said axis;
- and
- an elastomeric sealing member at least part of which is situated between said first compression face and said second compression face, said elastomeric sealing member being sized to fit slidably into the cartridge when said elastomeric sealing member is not compressed, said elastomeric sealing member capable of being compressed, between said first and second compression faces, by moving said second head piece toward the media bed so that said contact face of said first head piece presses against the stop, causing said elastomeric sealing member to expand laterally to form a seal with the cartridge.
The combination of a sealing head for a sample-conveying conduit and a chromatography cartridge comprising:
- a chromatography cartridge having walls;
- a chromatography media bed inside said cartridge forming a stop;
- a first head piece and a second head piece, both of which are elongated along a longitudinal axis and are sized to fit slidably into said cartridge, said first head piece having a body, a first compression face, and a contact face adapted to contact said stop, said second head piece having a second compression face and a compression force receiving member, said body defining an elongate flow channel along said axis;
- and
- an elastomeric sealing member at least part of which is situated between said first compression face and said second compression face, said elastomeric sealing member being sized to fit slidably into said cartridge when said elastomeric sealing member is not compressed, said elastomeric sealing member capable of being compressed, between said first and second compression faces, by moving said second head piece toward said media bed so that said contact face of first head piece presses against said stop and said elastomeric sealing member expands laterally to form a seal with the cartridge.
2 more elements
The combination of two sealing heads for sample-conveying conduits and a chromatography cartridge comprising:
- a chromatography cartridge having walls and having a first end and a second end;
- a media bed within said chromatography column forming first and second stops;
- a first sealing head having a first head piece and a second head piece, both of which are elongated along a first longitudinal axis and are sized to fit slidably into said first end of said cartridge, said first head piece having a first body, a first compression face, and a first contact face adapted to contact said first stop, said second head piece having a second compression face and a first compression force receiving member, said first body defining an elongate flow channel along said first axis, said first sealing head also having a first elastomeric sealing member at least part of which is situated between said first compression face and said second compression face, said first elastomeric sealing member being sized to fit slidably into said first end of said cartridge when said first elastomeric sealing member is not compressed, said first elastomeric sealing member capable of being compressed, between said first and second compression faces, by moving said second head piece toward said media bed so that said contact face of said first head piece presses against said first stop, causing said elastomeric sealing member to expand laterally to form a seal with said cartridge;
- and
- a second sealing head having a third head piece and a fourth head piece, both of which are elongated along a second longitudinal axis and are sized to fit slidably into said second end of said cartridge, said third head piece having a second body, a third compression face, and a second contact face adapted to contact said second stop, said fourth head piece having a fourth compression face and a compression force receiving member, said second body defining an elongate flow channel along said second axis, said second sealing head also having a second elastomeric sealing member at least part of which is situated between said third compression face and said fourth compression face, said second elastomeric sealing member being sized to fit slidably into said second end of said cartridge when said second elastomeric sealing member is not compressed, said second elastomeric sealing member capable of being compressed between said third and fourth compression faces, by moving said fourth head piece toward said media bed so that said second contact face of said third head piece presses against said second stop, causing said second elastomeric sealing member to expand laterally to form a seal with said cartridge.
2 more elements
A method for sealing a sample-conveying conduit to a chromatography cartridge containing a media bed that forms a stop comprising the steps of:
- providing a sealing head with a first head piece and a second head piece, both said first and second head pieces sized to fit slidably into an open end of the cartridge, said first head piece having a body, a first compression face, and a contact face adapted to contact the stop, said second head piece having a second compression face and a compression force receiving member;
- providing an elastomeric sealing member at least part of which is situated between said first compression face and said second compression face, said elastomeric sealing member being sized to fit slidably into the cartridge when not compressed;
- inserting said first head piece along its axis, said second head piece, and said elastomeric sealing member into the cartridge, while said elastomeric sealing member is in an uncompressed state;
- and
- compressing said elastomeric sealing member, between said first and second compression faces, by moving said second head piece toward the media bed so that contact face of said first head piece presses against the stop, causing said elastomeric sealing member to expand laterally to form a seal with the cartridge.
2 more elements
An apparatus for sealing a vessel containing a media that forms a stop comprising:
- a first head piece and a second head piece, both of which are sized to fit slidably into the vessel, said first head piece having a body, a first compression face, and a contact face adapted to contact the stop, said second head piece having a second compression face and a compression force receiving member;
- and
- an elastomeric sealing member at least part of which is situated between said first compression face and said second compression face, said elastomeric sealing member being sized to fit slidably into the vessel when not compressed, said elastomeric sealing member capable of being compressed, between said first and second compression faces, by moving said second head piece toward the media bed so that said contact face of said first head piece presses against the stop, causing said elastomeric sealing member to expand laterally to form a seal with the vessel.
An apparatus for sealing a sample-conveying conduit to a chromatography column comprising:
- a first head piece, a second head piece, and an intermediate head piece, all of which are elongated along a longitudinal axis and are sized to fit slidably into the column, said first head piece having a body, a contact face, said body defining an elongate flow channel along said axis;
- and
- first and second elastomeric sealing members, at least part of said first elastomeric sealing member situated between said first head piece and said intermediate head piece, at least part of said second elastomeric sealing member situated between said intermediate head piece and said second head piece, said elastomeric sealing members sized to fit slidably into the column when said elastomeric sealing members are not compressed, said elastomeric sealing members capable of being compressed, by the relative movement of any of said head pieces, so that said elastomeric sealing members expand laterally and form seals with the column.
A sealing apparatus for sealing a sample-conveying conduit to a chromatography column filled with a first media bed and having open ends comprising:
- a first head piece having a body, a shoulder, and a contact face, said contact face capable of transmitting pressure against the first media bed;
- a second head piece defining a first central opening through which said body is fit slidably;
- an intermediate head piece defining a second central opening through which said body is fit slidably, said intermediate head piece disposed between said shoulder and said second head piece;
- a plurality of elastomeric sealing members, including at least first and second elastomeric sealing member, said first elastomeric sealing member disposed between said shoulder and said intermediate head piece, and said second elastomeric sealing member disposed between said intermediate head piece and said second head piece.
1 more element
The combination of a sealing head for a sample-conveying conduit and a chromatography column comprising:
- a chromatography column having walls;
- a first head piece elongated along a longitudinal axis and sized to fit slidably into said column, said first head piece having a contact face and a body, said body defining an elongate flow channel along said axis;
- a second head piece slidably connected to said body of said first head piece;
- an intermediate head piece slidably connected to said body of said first head piece;
- and
- a plurality of elastomeric sealing members, including at least a first and second elastomeric sealing member, said first sealing member situated between said first head piece and said intermediate head piece, said second sealing member situated between said intermediate head piece and said second head piece, said first and second elastomeric sealing members being sized to fit slidably into said column when said elastomeric sealing members are not compressed, said first and second elastomeric sealing members capable of being compressed by the relative movement of said first and second head pieces, so that said elastomeric sealing members expand laterally.
3 more elements
A method for sealing a sample-conveying conduit to a chromatography column comprising the steps of:
- providing a sealing apparatus sized to fit slidably into an open end of the column, said sealing apparatus comprising a first head piece, a second head piece, an intermediate head piece, and a plurality of elastomeric sealing members, said second head piece and said intermediate head piece slidably connected to a body of said first head piece, said plurality of elastomeric sealing members including at least a first elastomeric sealing member and a second elastomeric sealing member, said first elastomeric sealing member disposed between said first head piece and said intermediate head piece, said second elastomeric sealing member disposed between said intermediate head piece and said second head piece;
- inserting said apparatus into the column, while said first and second elastomeric sealing members are in an uncompressed state;
- and
- compressing said first and second elastomeric sealing members, by moving said first and second head pieces relative to each other, thus causing said elastomeric sealing members to expand laterally.
1 more element
A chromatography sample module comprising
- a flow-through member having an inlet and an outlet,
- chromatography media within said flow-through member, and
- a sample carried on said media.
A chromatography sample module comprising a tubular member that is sized to fit within the end of a chromatography column, said module having an inlet and an outlet, and chromatography media within said tubular member.
The combination comprising
- a chromatography column having a module receiving region at an inlet end thereof, and
- a chromatography sample module located within said module receiving region, said module including a flow-through member having an inlet and an outlet, and chromatography media within said flow-through member.
A chromatography method comprising
- providing a chromatography sample module including a flow-through member having an inlet, an outlet, and chromatography media within said flow-through member,
- dissolving a sample in a solvent resulting in a dissolved sample,
- adding said dissolved sample to said media, and
- flowing solvent into said inlet and directing the effluent from said outlet to a chromatography column.
1 more element
Chromatography sample preparation apparatus comprising
- a plurality of chromatography sample modules, each said module including a flow-through member having an inlet, an outlet, and chromatography media within said flow-through member, and
- a support structure supporting said plurality of modules.
Dependencies are inferred from the claim text.