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US 2002/0153252 A1Published application
Multi-dimensional proteomic analysis method
OwnerRobert E. Akins JR.inventor
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Abstract
A multi-dimensional proteomic analysis method utilizing cationic electrophoresis is described. The method includes separating proteins in one direction using cationic electrophoresis and separating the proteins in a second orthogonal direction using other electrophoresis separation methods such as denaturing electrophoresis and electrophoresis subsequent to proteolytic cleavage or isofocussing. The two dimensional array may be used to determine various protein-protein interactions in a sample.
Claims10
A multi-dimensional proteomic analysis method comprising the steps of:
- separating a protein sample on a substrate by cationic electrophoresis in a first direction on the substrate;
- and
- separating the protein sample on the substrate by a second electrophoresis method in a second direction substantially orthogonal to the first direction to produce a two-dimensional array of separated proteins.
A multi-dimensional proteomic analysis method comprising the steps of:
- separating a peptide sample on a substrate by cationic electrophoresis in a first direction on the substrate;
- and
- separating the peptide sample on the substrate by a second electrophoresis method in a second direction substantially orthogonal to the first direction to produce a two-dimensional array of separated peptides.
Dependencies are inferred from the claim text.