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AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorsDavid Alland, Barry R. Bloom, William R. Jacobs JR.
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This invention relates to the identification, cloning, sequencing and characterization of the iniB, iniA and iniC genes of mycobacteria which are induced by a broad class of antibiotics that act by inhibiting cell wall biosynthesis, including the first line antituberculosis agents, isoniazid and ethambutol. The present invention provides purified and isolated iniB, iniA, iniC and iniB promoter nucleic acids which may comprise the iniBAC operon, as well as mutated forms of these nucleic acids. The present invention also provides one or more single-stranded nucleic acid probes which specifically hybridize to the iniB, iniA, iniC and iniB promoter nucleic acids, and mixtures thereof, which may be formulated in kits, and used in the diagnosis of drug-resistant mycobacterial strain. The present invention also provides methods for the screening and identification of drugs effective against Mycobacterium tuberculosis using induction of the iniB promoter.
A purified and isolated nucleic acid sequence of the iniA gene.
A single-stranded nucleic acid probe which specifically hybridizes to a nucleic acid sequence of the iniA gene.
A purified, active protein encoded by the iniA gene.
An antibody immunoreactive with a protein encoded by the iniA gene.
A purified and isolated nucleic acid sequence of the iniB gene.
The nucleic acid sequence of claim 18 which is obtained from M. tuberculosis.
A single-stranded nucleic acid probe which specifically hybridizes to a nucleic acid sequence of the iniA gene.
A purified, active protein encoded by the iniB gene.
An antibody immunoreactive with a protein encoded by the iniB gene.
A purified and isolated nucleic acid sequence of the iniC gene.
A single-stranded nucleic acid probe which specifically hybridizes to a nucleic acid sequence of the iniC gene.
A purified, active protein encoded by the iniC gene.
An antibody immunoreactive with a protein encoded by the iniC gene.
A vector construct comprising the nucleotide sequence of the iniB promoter inserted into a plasmid.
A method of determining whether a drug is effective against Mycobacterium tuberculosis comprising:
Dependencies are inferred from the claim text.